文章摘要
施文瑞,王磊,方冬冬,李梦荣,李学军,陈军平,刘慧芬,董传举.基于线粒体COⅠ基因序列的大鳞副泥鳅四个群体遗传多样性[J].水产学报,2020,44(2):179~186
基于线粒体COⅠ基因序列的大鳞副泥鳅四个群体遗传多样性
Genetic diversity based on the mitochondrial COⅠ gene sequences in four populations of Paramisgurnus dabryanus
投稿时间:2018-09-16  修订日期:2019-04-26
DOI:10.11964/jfc.20180911456
中文关键词: 大鳞副泥鳅  COⅠ基因  遗传多样性
英文关键词: Paramisgurnus dabryanus  COⅠ gene  genetic diversity
基金项目:国家自然科学基金(31602149);河南师范大学博士科研启动基金(qd14184)
作者单位E-mail
施文瑞 河南师范大学水产学院, 河南 新乡 453007  
王磊 河南师范大学水产学院, 河南 新乡 453007 hnnuwl@163.com 
方冬冬 河南师范大学水产学院, 河南 新乡 453007  
李梦荣 河南师范大学水产学院, 河南 新乡 453007  
李学军 河南师范大学水产学院, 河南 新乡 453007  
陈军平 河南师范大学水产学院, 河南 新乡 453007  
刘慧芬 河南师范大学水产学院, 河南 新乡 453007  
董传举 河南师范大学水产学院, 河南 新乡 453007  
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中文摘要:
      为了解大鳞副泥鳅群体遗传多样性现状,实验利用线粒体COI基因部分序列对来自于辽宁、河南、湖北和台湾的大鳞副泥鳅4个群体进行了遗传多样性分析。结果显示,在642 bp的COI基因序列中,碱基A、T、C、G的平均含量分别为23.5%、30.6%、26.7%、19.2%,表现出较强的AT偏好性;4个群体共检测到44个多态性位点,定义了23个单倍型;4个群体的单倍型多样性指数(h)为0.424~0.855,核苷酸多样性指数(π)为0.000 84~0.016 59,台湾群体遗传多样性较高,河南群体次之,湖北群体和辽宁群体较低;群体间遗传分化系数FST及遗传距离分析表明,台湾群体与其他群体间均存在极显著的遗传分化,且与其他群体间的遗传距离均较远;AMOVA分析结果表明,群体间的遗传变异高于群体内的变异(52.11%>47.89%);辽宁、河南和湖北群体的中性检验Tajima’s D及Fu’s Fs均为负值,提示辽宁、河南和湖北群体可能经历过群体爆发和扩张事件。研究表明,大鳞副泥鳅不同地理群体形成了一定的遗传分化,研究结果为大鳞副泥鳅的遗传多样性保护及选育等工作提供了依据。
英文摘要:
      In order to research the genetic diversity of different populations of Paramisgurnus dabryanus, four populations from Liaoning, Henan, Hubei and Taiwan were analyzed by sequencing the mitochondrial cytochrome oxidase subunit Ⅰ(COⅠ) genes. The results showed that 642 base pairs (bp) fragments was consisted of A, T, C and G base with 23.5%, 30.6%, 26.7% and 19.2%, respectively, indicating a preference for A and T bases. A total of 44 mutations of nucleotide and 23 haplotypes were identified in 4 populations. The haplotype diversity index ranged from 0.424-0.855, and the nucleotide diversity ranged from 0.000 84-0.016 59. The results indicated that Paramisgurnus dabryanus from Taiwan population had a higher genetic diversity than Liaoning, Henan and Hubei populations. The genetic differentiation index (Fst) and genetic distance showed that genetic differentiation between Taiwan and other populations represents extremely significant difference, also had a further genetic distance with other populations. In general, the results showed that there was a certain genetic difference between the different geographical groups, AMOVA analysis revealed that 52.11% of genetic variations derived among populations and 47.89% of genetic variations occurred within populations. In terms of the negatively selective neutrality test, the results indicated that a population expansion occurred in the populations of Liaoning, Henan and Hubei population, and provided a reference for protection of the genetic diversity and breeding work of Paramisgurnus dabryanus.
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