文章摘要
郝甜甜,王际英,马长兴,周莹,李宝山,王成强,孙永智,黄炳山.硒对铜胁迫下大菱鲆幼鱼生长、抗氧化能力及相关基因表达的影响[J].水产学报,2019,43(11):2304~2316
硒对铜胁迫下大菱鲆幼鱼生长、抗氧化能力及相关基因表达的影响
Effects of dietary selenium levels on growth performance, antioxidant capacity and related gene expression of juvenile turbot (Scophthalmus maximus) under copper stress
投稿时间:2018-10-30  修订日期:2018-12-27
DOI:10.11964/jfc.20181011515
中文关键词: 大菱鲆  铜胁迫    生长  抗氧化能力  基因表达
英文关键词: Scophthalmus maximus  copper stress  selenium  growth  antioxidant capacity  gene expression
基金项目:烟台市科技计划(2018ZHGY066)
作者单位E-mail
郝甜甜 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
王际英 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006 ytwjy@126.com 
马长兴 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006
上海海洋大学, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种上海市协同创新中心, 上海 201306 
 
周莹 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006
上海海洋大学, 水产科学国家级实验教学示范中心, 农业农村部鱼类营养与环境生态研究中心, 水产动物遗传育种上海市协同创新中心, 上海 201306 
 
李宝山 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
王成强 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
孙永智 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
黄炳山 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 山东 烟台 264006  
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中文摘要:
      为探讨硒对铜胁迫下大菱鲆幼鱼生长、抗氧化能力及相关基因表达的影响,以初始体质量为(24.85±0.10) g的大菱鲆为研究对象,通过在饲料中添加五水硫酸铜和亚硒酸钠,配制铜、硒含量分别为(0、0,1 000、0,1 000、2,1 000、4 mg/kg) 4种等氮等能的实验饲料,命名为D1、D2、D3和D4组。养殖实验持续84 d。结果显示:①各组间实验鱼成活率无显著差异;增重率、特定生长率和蛋白质效率均在D2组显著低于D1、D3和D4组;摄食率和饲料系数呈相反趋势,在D2组显著高于其他3组。②幼鱼全鱼和肝脏粗脂肪含量呈先下降后上升趋势;而背肌粗蛋白和粗脂肪含量在各组之间无显著性差异。全鱼、脊椎骨和肝脏中Cu含量在D2组达到最高值;Zn含量则在D4组达到最高值;肝脏中铁含量呈下降趋势,在D3和D4组显著低于D1和D2组。③D2组显著降低了幼鱼血清总抗氧化能力(T-AOC)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性;丙二醛(MDA)含量则呈相反趋势,在D2组显著高于D3和D4组;总超氧化物歧化酶(T-SOD)和铜—锌超氧化物歧化酶(CuZn-SOD)活性均在D4组达到最低值。④血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性均在D2组显著高于其他3组;而血糖浓度、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)活性均在D2组达到最低值,显著低于D1组。⑤肝脏金属硫蛋白(MT) mRNA相对表达量在D4组达到最高值,显著高于其他3组;谷胱甘肽转移酶(GST)和溶菌酶(LZM) mRNA相对表达量均在D2组显著低于其他3组;热休克蛋白70 (HSP70) mRNA相对表达量则在D2组显著高于其他3组。综上所述,在本实验条件下,饲料中添加硒(2~4 mg/kg)可缓解高铜(1 000 mg/kg)胁迫导致的鱼体生长缓慢等症状,并可调控鱼体的抗氧化能力、生理代谢及相关基因表达量,进而对鱼体在高铜胁迫下的内环境稳态恢复具有一定的促进作用。
英文摘要:
      The study was carried out to investigate the effect of dietary selenium(Se) supplementation on the growth performance, serum antioxidant enzyme activity and expression of related genes in liver of juvenile turbot (Scophthalmus maximus) under copper(Cu) stress. Four experimental diets(D1,D2,D3,D4) were formulated to contain the following diets with increment levels of copper 0, 1 000, 1 000, 1 000 mg/kg and selenium 0, 0, 2, 4 mg/kg. The group D1 as the control. Each diet was randomly assigned to triplicate groups of 40 fish [initial weight, (24.85±0.1) g] per aquarium. Fish were fed twice daily(8:30 and 16:30) at ratio of 1.5% or 2% body weight for 84 days. Results showed that the different diets had no significant effect on the survival rate of turbot. The weight gain rate, specific growth rate and protein efficiency ratio of D2 group were significantly lower than other groups. Whereas, feed conversion ratio and feed intake reached the maximum in group D2. Crude lipid content of whole body and liver all showed a trend of first decreasing and then rising, and, there were no significant differences of crude lipid and crude protein contents in muscle among all groups. Cu concentration of D2 group in whole body, vertebra and liver was remarkably higher than the others. Zn concentration in whole body, vertebra and liver reached the maximum in the group D4. Hepatic Fe concentration was lower in group D4 than the others. The serum total antioxidant activity, catalase activity, glutathione peroxidase activity decreased in group D2, and increased with further increase of selenium level. While the malondialdehyde content followed the opposite trend. Total superoxide dismutase and CuZn superoxide dismutase activity both reached the minimum in group D4. Both alanine aminotransferase and aspartate transaminase activities showed their highest values in group D2, which were significantly higher than those in the other groups. Glucose, triglyceride, total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol showed a opposite trend to alanine aminotransferase and aspartate transaminase activities with the lowest value in group D2, significantly lower than that in the control group. Metallothionein expression increased in the liver with the dietary selenium levels under Cu stress, which was significantly higher in group D4 than that in the other groups. Glutathione transferase and lysozyme expression levels decreased initially and then increased with the dietary change, which were significantly lower in group D2 than those in the control group. Heat shock protein 70(HSP70) expression level showed the highest values in group D2, which was significantly higher than those in the other groups. In conclusion, adequate dietary selenium levels could affect the growth performance, increase the total antioxidant capacities, modulate some mRNA expression of gene in liver, and then reduce the metabolic disorders caused by high copper content in juvenile S. maximus.
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