文章摘要
黄林彬,黄文,严兴洪.条斑紫菜两个耐高温品系的耐低盐特性[J].水产学报,2020,44(2):222~233
条斑紫菜两个耐高温品系的耐低盐特性
Low-salinity tolerance of two high-temperature resistant strains in Pyropia yezoensis
投稿时间:2019-03-12  修订日期:2019-05-15
DOI:10.11964/jfc.20190311687
中文关键词: 条斑紫菜  叶状体  叶绿素荧光  耐高温  耐低盐  逆境胁迫
英文关键词: Pyropia yezoensis  blade  chlorophyll fluorescence  high-temperature resistance  low-salinity tolerance  abiotic stress
基金项目:国家自然科学基金(31302185,31072208);国家重点研发计划(2018YFD0900606);浙江省农业新品种选育重大科技专项(2016C02055-6);江苏省科技计划(现代农业) (BE2018335)
作者单位E-mail
黄林彬 上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306 
 
黄文 宁德海洋环境监测中心站, 福建 宁德 352100  
严兴洪 上海海洋大学, 水产种质资源发掘与利用教育部重点实验室, 上海 201306
上海海洋大学, 水产科学国家级实验教学示范中心, 上海 201306
上海海洋大学, 国家海洋生物科学国际联合研究中心, 上海 201306 
xhyan@shou.edu.cn 
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中文摘要:
      以条斑紫菜两个耐高温品系(YZ-4和TM-18)为对象,以野生型品系(WT)做对照,分析其叶状体在高温、低盐胁迫下的生长和光系统Ⅱ最大光量子产量(Fv/Fm)的数值,为筛选既耐高温又耐低盐的条斑紫菜抗逆品系提供依据。结果显示,在适宜培养条件(温度18 ℃和盐度26)下,YZ-4和TM-18叶状体在50~85 d的生长速率快于WTFv/Fm和主要光合色素含量均高于WT,各品系的绝对生长率和Fv/Fm之间均具有较高的正相关性。另外,WT叶状体在70 d左右开始形成精子囊时,绝对生长率和Fv/Fm明显下降。在高温胁迫下培养,各品系叶状体的Fv/Fm和绝对生长率均呈下降趋势,且胁迫的温度越高或时间越长,其降幅越大。在24 ℃高温胁迫35 d后,WTYZ-4和TM-18的Fv/Fm分别下降了56.7%、43.2%和28.7%。在25 ℃高温胁迫15 d时,WT的生长完全停滞,而YZ-4和TM-18则分别保持0.51和0.84 cm/d的绝对生长率,说明二者确实是耐高温的。低盐胁迫对叶状体的影响与高温胁迫的类似,在盐度为9的低盐下培养35 d后,WTYZ-4和TM-18的Fv/Fm降幅分别为46.2%、42.0%和32.0%,三者的绝对生长率分别为0.12、0.10和0.90 cm/d。另外,WTYZ-4在盐度为5时生长受到明显影响,叶状体在15 d时颜色加深、卷曲变硬并腐烂,而TM-18在相同盐度下培养25 d也未腐烂,Fv/Fm仍高达初始值的72.8%。这表明TM-18在高温或低盐胁迫下的耐受性比WTYZ-4的都强,其内在原因是叶状体Fv/Fm的降幅较小,间接说明Fv/Fm可作为条斑紫菜抗逆品系选育的新指标。
英文摘要:
      The growth and maximum photon quantum yield of photosystem II (Fv/Fm) of the blades of two high-temperature resistant strains (YZ-4 and TM-18) and one wild-type strain (WT, used as control group) in Pyropia yezoensis were analyzed at high-temperatures or low-salinities, in order to screen out strains resistant to the two abiotic stresses. The results showed that YZ-4 and TM-18 blades grew faster than those of WT for 50-85 d under the optimal culture conditions (18℃ and salinity of 26). The ratios of Fv/Fm and contents of photosynthetic pigments were also higher than those of WT. The absolute growth rate and Fv/Fm of every strain showed high positive correlations. In addition, WT blades began to form spermatangia around 70 d, their absolute growth rate and Fv/Fm decreased significantly. When cultured at high-temperatures, Fv/Fm and absolute growth rates of the blades of every strain decreased. The higher the temperature or the longer the stress lasted, the greater the decline would be. Fv/Fm of WT, YZ-4 and TM-18 decreased by 56.7%, 43.2% and 28.7%, respectively, after 35 d of high-temperature stress at 24℃. The growth of WT was completely stagnated after 15 d of high-temperature stress at 25℃. Meantime, growth rates of YZ-4 and TM-18 were 0.51 and 0.84 cm/d, respectively, indicating that they were veritable high-temperature resistant strains. The effects of low-salinity stress on the blades were similar to those of high-temperature stress. After 35 d of low-salinity stress at salinity of 9, Fv/Fm of WT, YZ-4 and TM-18 decreased by 46.2%, 42.0% and 32.0%, respectively. The absolute growth rates of WT, YZ-4 and TM-18 were 0.12, 0.10 and 0.90 cm/d, respectively. Besides, growth of the blades of WT and YZ-4 cultured at salinity of 5 was greatly affected, in that the blades were deepened in color, curled and rotted after 15 d under stress, while TM-18 did not rot at the same salinity of 5 for 25 d. These results indicated that TM-18 was more tolerant to high-temperature and low-salinity stress than WT and YZ-4, and the internal reason was that the decrease of Fv/Fm of TM-18 blades was small. It indicated indirectly that Fv/Fm could be used as a new index for breeding of stress-resistant strains in P. yezoensis.
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