文章摘要
陈书健,吴成龙,叶金云,李荣华.饲料中维生素A对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢酶活性及基因表达的影响[J].水产学报,2020,44(1):85~98
饲料中维生素A对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢酶活性及基因表达的影响
Effect of dietary vitamin A on growth, serum biochemical index, digestive enzyme activities and glucose and lipid metabolism in juvenile Mylopharyngodon piceus
投稿时间:2019-05-13  修订日期:2019-05-28
DOI:10.11964/jfc.20190511785
中文关键词: 青鱼  维生素A  生化指标  糖脂代谢  基因表达
英文关键词: Mylopharyngodon piceus  vitamin A  biochemical index  glucose and lipid metabolism  gene expression
基金项目:国家大宗淡水鱼产业技术体系岗位科学家任务(CARS-45-11);国家自然科学基金面上项目(31672669)
作者单位E-mail
陈书健 宁波大学海洋学院, 宁波大学应用海洋生物学教育部重点实验室, 浙江 宁波 315211
湖州师范学院生命科学学院, 浙江省水生生物资源养护与开发技术研究重点实验室, 浙江 湖州 313000 
 
吴成龙 湖州师范学院生命科学学院, 浙江省水生生物资源养护与开发技术研究重点实验室, 浙江 湖州 313000  
叶金云 湖州师范学院生命科学学院, 浙江省水生生物资源养护与开发技术研究重点实验室, 浙江 湖州 313000 ziff2006@163.com 
李荣华 宁波大学海洋学院, 宁波大学应用海洋生物学教育部重点实验室, 浙江 宁波 315211  
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中文摘要:
      为研究饲料中维生素A(VA)对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢相关酶活性及基因表达的影响,实验选取360尾初始体质量为(6.10±0.10) g的青鱼幼鱼,随机分配至3个实验组中,每个实验组设置3个平行。采用单因素实验设计,以无维酪蛋白和明胶为蛋白源、菜籽油为脂肪源、糊精为糖源,同时添加矿物质混合物和维生素混合物(无VA添加)配制成3组实验饲料,分别以饲料1 (Diet1)、饲料2 (Diet2)和饲料3 (Diet3)表示。在饲料1、饲料2和饲料3中分别添加0、2 200和20 000 IU/kg VA醋酸酯(500 000 IU/g),经高效液相色谱法(Agilent-1100, Agilent, 美国)检测后实验饲料中VA的实际含量分别为178.2、2 058.9和18 436.2 IU/kg,养殖周期为8周。结果显示:饲料中VA缺乏会显著降低青鱼幼鱼的增重率(WGR)和特定生长率(SGR);VA缺乏会显著降低血清血糖(GLU)、甘油三酯(TG)和低密度脂蛋白(LDL)浓度,增加总胆固醇(TCH)浓度。饲料中添加2 058.9 IU/kg VA能显著提高肝脏己糖激酶(HK)、磷酸果糖激酶(PFK)、丙酮酸激酶(PK)活性,促进葡萄糖转运蛋白-2 (GLUT-2)、HK、葡萄糖激酶(GK)、PFK和葡萄糖-6-磷酸酶(G6Pase)基因表达。当饲料中VA含量为2 058.9 IU/kg时,对肝脏脂肪酸转运蛋白-1 (FATP-1)基因表达无显著影响,但显著影响肉碱棕榈酰转移酶-1 (CPT-1)和肉碱棕榈酰转移酶-2 (CPT-2)基因表达。当饲料中VA缺乏时,CPT-1和CPT-2基因表达受到显著性抑制;当饲料中添加过量VA时,乙酰辅酶A羧化酶-2 (ACC-2)和脂蛋白酯酶(LPL)基因表达受到抑制;同时,VA过量组中肝脏过氧化物酶体增殖物激活受体-γ (PPAR-γ)基因表达显著下降。研究表明,在饲料中添加2 058.9 IU/kg VA可以促进青鱼幼鱼生长,提高肝脏对葡萄糖的转运能力,促进糖酵解和糖异生代谢平衡,同时促进脂肪酸合成和转运。
英文摘要:
      An 8-week feeding trial was carried out to investigate the effect of dietary vitamin A (178.2, 2 058.9, 18 436.2 IU/kg) on growth performance, serum biochemical index and hepatic glucose and lipid metabolism key enzyme activities and gene expression levels of juvenile black carp (Mylopharyngodon piceus). Using a one-factor experiment design, three isonitrogenous and isoenergetic purified experimental diets (diet 1, diet 2 and diet 3) containing 0, 2 200 and 20 000 IU/kg VA were formulated. Total vitamin A contents in the diets were measured by HPLC (Agilent-1100, Agilent, USA), actual content of vitamin A in each diet were 178.2, 2 058.9 and 18 436.2 IU/kg, respectively. Casein (vitamin free) and gelatin were used as protein sources, rapseed oil was used as lipid source, dextrin was used as carbohydrate source. Total 360 juvenile black carp with initial weight (6.10 ±0.10) g were randomly divided into 3 groups with 3 replicates per group and 40 black carp per replicate. The results showed as follows: weight gain rate (WGR) and specific growth rate (SGR) were significantly reduced for fish fed diet with 178.2 IU/kg VA. When VA is deficient, concentrations of serum glucose (GLU), triglycerides and low-density lipoprotein (LDL) were decreased, but total cholesterol (TCH) concentration was increased. The activities of hexokinase (HK), phosphofructokinase (PFK) and pyruvate (PK) increased for fish fed diet with 2 058.9 IU/kg VA. Gene expression of liver glucose transporter-2 (GLUT-2), HK, GK, PFK and G6Pase were increased in fish fed diet with 2 058.9 IU/kg VA. However, there were no significant differences in the gene expression of fatty acid transporter protein-1 (FATP-1) for fish fed diet with 2 058.9 IU/kg VA. But the gene expression of Carnitine O-palmitoyltransferase-1 (CPT-1) and Carnitine O-palmitoyltransferase-2 (CPT-2) were significantly influenced. The gene expression of CPT-1 and CPT-2 were inhibited for fish fed VA deficient diet. When fish fed diet with 18 436.2 IU/kg VA, the expression of acetyl-CoA carboxylase-2 (ACC-2) and lipoprotein lipase (LPL) genes were inhibited. Besides, the gene expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) decreased when the VA content was 18 436.2 IU/kg in feed. Overall, fish fed diet with 2 058.9 IU/kg VA could improve growth performance and ability of liver cells to transport glucose of juvenile black carps. Diet with 2 058.9 IU/kg VA could keep a balance between glycolysis and gluconeogenesis, and it also could promote fatty acids synthesis and transport.
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