文章摘要
刘沛霖,曹杰超.单尾金鱼脑垂体切除的方法[J].水产学报,1965,2(2):71~75
单尾金鱼脑垂体切除的方法
A MODIFIED METHOD OF HYPOPHYSECTOMY IN THE GOLD FISH,Carassius auratus(L.)
  
DOI:
中文关键词: 黑龙江  银鲫  单性  性反转  无性生殖
英文关键词: 
基金项目:
作者单位
刘沛霖 中国科学院水生生物研究所 
曹杰超 中国科学院水生生物研究所 
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中文摘要:
      在研究脑垂体或脑垂体激素的生理功能时,如果不把实验动物本身的脑垂体预先除去,就很难避免内源激素的影响,往往不能作出令人信服的结论。因此,切除脑垂体是研究脑垂体内分泌生理最常用的一种重要技术。 根据Pickford氏(1957)的记载从1911年以来已有19种硬骨鱼作脑垂体切除,这些手术大致都是从口腔顶或鳃腔剥除部分副蝶骨或在副蝶骨钻孔而切除的。这样的方法正如Chavin指出的往往不可避免的会损伤周围的血管特别是内颈动脉。
英文摘要:
      A modified approach of hypophysectomy in the goldfish has been suggested. The main fea-ture of this method is that the pituitary gland is taken out from the opening made by cuttingthrough the anterior parl of the prootic instead of drilling through the parasphenoid bone onthe median oral roof. An incision about 3 mm. long was made through the opercular cavity onthe covering of the oral roof behind the eye. The opercular adductor muscle thus revealed wasexcised. By using a sharp fine scalpel the anterior portion of the prootic and the pterggoidangle of the parasphenoid were cut away. By pushing a little aside the external rectus muscleof the eye and the palatine branch of the Ⅵ eranial nerve, the pituitary gland was exposedand could be sucked away by a fine pipette of suitable size. The operation could be done within 5-7 minutes without doing any injury to the main ves-sels or nerves. Mortality after operation did not exceed 15% of fish operated at 25℃. The operation could be performed either by holding the fish in one hand and doing theoperation with the other or by fixing the fish on a specially designed operation board. Thisboard could also be used in operations other than hypophysectomy in fishes.
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