文章摘要
张念慈,杨广智.草鱼吻端组织细胞株ZC-7901及其亚株ZC-7901S1的建立和特性观察[J].水产学报,1981,5(2):111~120
草鱼吻端组织细胞株ZC-7901及其亚株ZC-7901S1的建立和特性观察
THE ESTABLISHMENT OF STRAIN ZC-7901 AND SUBSTRAIN ZC-7901S_1 FROM THE SNOUT TISSUE CELL8 OF GRASS CARP
  
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作者单位
张念慈 浙江省淡水水产研究所 
杨广智 浙江省淡水水产研究所 
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中文摘要:
      以草鱼吻端组织为材料,经体外培养建立了一个上皮样细胞和梭形细胞混合的细胞株,后又从中分离出一个上皮样细胞亚株,分别定名为ZC-7901和ZC-7901S1。对两株细胞进行了基本生物学特性的测定,染色体数目和经长期传代后染色体数目稳定性的分析。实验表明,两株细胞为草鱼正常二倍体(2n=48)细胞株。
英文摘要:
      By using the technic of tissue culture we have successfully established a strain ofcomplex of epitheial-like and fibroblast-like cells from the snout tissue of grass carp(Ctenopharyngodon idellus), and it is denoted as strain ZC--7901. The medium used inthe cultivation is"199" supplemented with 15% fetal bovine serum containing a mode-rate amount of antibiotics. The incubation temperature is at 25--28℃. A substrain ofsingle epitheial-like cells is also obtained from ZC--7901 cells of 11th generation, whichis named substrain ZC--7901S_1. At Present both the strain and substrain have been maintained in proliferationfor over 22 months through 120 and 90 generations respectively, and the cells ofthem are able to reproduce with approximately 1.5×10~5 cells per millilitre in con-centration. About 4 days, a monolayer cells can be found clearly in the culture. After36 hours, the index of mitotic division of the ZC-7901 is 36.5‰, while that of theZC-7901S_1 is 32.3‰; and then at 156 hours after propagation the former is gradullydecreased to 12.3‰ and the latter to 14.3‰. The chromosome number of each cell inthese two strains is 2n = 48.
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