文章摘要
赵焕登,张学成.条斑紫菜营养细胞的分离培养试验[J].水产学报,1984,8(3):197~202
条斑紫菜营养细胞的分离培养试验
ON THE CULTIVATION OF THE ISOLATED VEGETATIVE CELLS OF PORPHYRA YEZOENSIS UEDA
  
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赵焕登 山东海洋学院生物系 
张学成 山东海洋学院生物系 
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中文摘要:
      本文介绍了用条斑紫菜的单个营养细胞培养成叶状体的试验。试验表明,单个营养细胞,在适宜的培养液中有可能经过20天培养成小紫菜叶状体;其成活率为33%左右。此外,还介绍了冷冻度夏后的叶状体单个营养细胞的培养试验和不同含水量的叶状体营养细胞的培养试验。
英文摘要:
      Individual vegetative cells of Porphyra yezoensis were obtained by mechanical smashing, The isolated cells were oultured In enriched soawster and developed into new fronds in 20 days. The survial rate was about 33%.In attemping to make use of the tatipotency of the vegetative cells of this alga to meet the ever increasing demand for seedlings in commercial culture.A series of experiments were carried out involving the freezing of the seaweed and keeping it at -20℃ for six months from June till December. Then the fronds were smashed to obtain the isolated vegetative cells, which could grow into normal frond seither in the laboratory or in natural sea waters.Results of the experiments revealed tb.at the survival rats of isolated cells is directly related to the water content of the fronds before freezing. When the water content 50% the survival rate was highest in this experiment.It seems possible by appling this principle produce great amount of seedlings to meet commercial need.
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