文章摘要
徐根兴,朱洪文.鲫鱼尾垂体抽提液对鳜鱼离体肠段的收缩效应[J].水产学报,1987,11(1):10~16
鲫鱼尾垂体抽提液对鳜鱼离体肠段的收缩效应
EXTRACTS OF UROPHYSIS OF CRUCIAN CARP INDUCING CONTRACTION EFFECT ON THE INTESTINES OF MANDARIN FISH IN VITRO
  
DOI:
中文关键词: 尾垂体  鲫鱼  鳜鱼  收缩效应  
英文关键词: Urophysis  Crucian carp  Mandarin fish  Contraction effect  Intestines
基金项目:
作者单位
徐根兴 南京大学生物系 
朱洪文 南京大学生物系 
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中文摘要:
      鲫鱼尾垂体抽提液,可引起鳜鱼离体肠段或肠段纵行肌的节律性收缩。它的收缩效应具高度敏感性井和剂量相关。每毫升浸液0.05个尾垂体就能引起可重复的收缩反应。因此,我们建议用分离的鳜鱼肠段来取代鲑鳟鱼的直肠,以建立尾垂体紧张素II的生物测定方法。乙酰胆碱可作为实验室参考标准。
英文摘要:
      Extracts of urophysis of crucian carp (Carussius auratus L.)induced rhythmic contractions of the intestines of mandarin fish (Siniperca chuatsiB.)in vitro. The contractfion effect of the extracts showed a high degree of sensitivityand good reproducibility. The experiments was found to be dose-dependent. Repro-ducible responses were obtained with as little as 0.05 of a urophysis/ml in fluid bath.It was suggested that the isolated mandarin fish intestines could be used to substitutefor trout rectum in order to provide the bioassay method for the estimation of urote-nsin Ⅱ. Acetylcholine Was used for a laboratory reference standard.
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