文章摘要
赵维信.虹鳟排卵前后血清中性类固醇激素浓度变化的研究[J].水产学报,1987,11(3):205~213
虹鳟排卵前后血清中性类固醇激素浓度变化的研究
CHANGES OF SERUM STEROID LEVELS DURING OVULATION IN RAINBOW TROUT(SALMO GAIRDNERI)
  
DOI:
中文关键词: 虹鳟  血清类固醇激素  诱导排卵  鲑 GnRⅡ及其类似物
英文关键词: Rainbow trout  Seram steroid  Induced ovulation  Salmon GnRH and its analogue
基金项目:
作者单位
赵维信 上海水产大学 
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中文摘要:
      对虹鳟(Salmo gairdneri)排卵前后血清17β-雌二醇(17β-E2),睾酮(T)和17α-羟-20β-双氢孕酮(17α20βP)的含量进行了测定。17β-E2从排卵前15天的18.1ng/ml急骤下降到排卵前3—6天的2ng/ml,并继续下降,至排卵时仅为0.9ng/ml。睾酮在虹鳟雌鱼血清中的含量很高,血清浓度从排卵前9天的峰值水平143.3ng/ml缓慢下降,排卵时为24.3ng/ml。17α20βP的血清浓度变化明显,排卵前15天,该激素浓度接近于基线水平或甚至低得不能被检测,排卵前9天开始迅速上升,到排卵前3天达到峰值350.6ng/ml,排卵时为302.2ng/ml。本研究进一步证实了17α20βP在鲑鳟鱼类卵母细胞最后成熟过程中的生理作用,是一种诱发卵母细胞成熟的类固醇激素。临排卵前,血清17β-E2浓度的下降,可能调节了17α20βP大量分泌的时间;17α20βP的大量分泌是卵母细胞达到最后成熟和排卵不可缺少的一环。注射合成的大麻哈鱼(Oncorhynchus keta)促性腺激素释放激素(s-GnRH)及其类似物(s-GnRH-A),诱发虹鳟血清类固醇激素17β-E2,睾酮和17α20βP的变化趋势与自然排卵时的变化相类似。经注射药物诱发排卵的鱼,较对照组提早一周排卵,而且排卵较同步和集中。
英文摘要:
      Serum levels of 17β-oestradiol (17β-E2.), testercon? (T) and17α-hydroxy-20β-dihydropogesterone (17α20βP) were measeured in female rainbowtrouts (Salmo gairdneri) during their first ovulation. 17β-E2 fell from 18.1 ng/ml15 days to 2ng/ml 3 to 6 days prior to ovulation, and it was 0.9ng/ml duringovulation. Testosterone reached to a peak 1evel of 143. 3ng/ml ct 9 days prior to ovula-tion, then the level fell to 24. 3ng/ml during ovulation. At 15 days before ovulation,17α 20β P levels were either nondetectable or extremely low, but it rose rapidly from9 days and reached to a peak level of 350.6ng/ml at 3 days before ovulation. Whileovulating the level become to 302.2ng/ml. This work provides further evidence for17α 20β P that plays an important role in final maturation of oocyto. 17β-E2. maypossibly regulate the timing of 17α 20β P surge. Injection of synthetic chum salmon (Oncorhychus keta)gonadotrophin-releasinghormone (s-GnRH) and its analogue (s-GnRH-A), the changes of serum 17β-E2, Tand 17α 20β P levels are similar to that of natural ovulation. s-GnRH and s-GnRH-Ainjected fish ovulated a week earlier and synchronous than those of control.
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