文章摘要
黄志斌,李淡秋.快速制备脂肪酸甲酯用于气相色谱分析[J].水产学报,1990,14(1):40~43
快速制备脂肪酸甲酯用于气相色谱分析
A RAPID PREPARATION OF FATTY ACID METHYL ESTERS FOR GAS CHROMATOGRAPHIC ANALYSIS
投稿时间:1990-03-01  修订日期:1990-03-01
DOI:
中文关键词: 快速制备  脂肪酸甲酯  气相色谱分析
英文关键词: rapid preparation  fatty acid methyl ester  gas chromatographic analysis
基金项目:
作者单位
黄志斌 上海水产大学 
李淡秋 上海水产大学 
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中文摘要:
      在不需从原料中提取脂质,而直接将磨碎原料中的脂质皂化、甲酯化后,进行气相色谱的脂肪酸分析测定。这种方法与通常采用的先提脂质再行甲酯化的方法进行了比较,结果表明,分析测定的数据与普通法极为一致,其优点是快速、操作方便、节省药品;即使样品很少,用普通法不易提取脂质时,此法也能顺利地完成分析工作。
英文摘要:
      A rapid preparation of fatty acid methyl esters by using direct reactionin the ground sample without lipid extraction was developed. Fish flesh, fish viscera,fish liver. fish eggs and dried shrimps were used as samples in comparative experi-ments. The results of this new direct method were extremely close to those of or-dinary method in which lipid was extracted according to Bligh-Dyer's method andfatty acids was esterified according to Metcalfe's method (tt_(0.05). The advantagesof this direct method are rapid, reagent saving, simple and convenient. It can solvethe difficulty in extracting lipid from samples of small amount or of less in lipid inthe case of ordinary method.
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