文章摘要
郑国兴,李何,黄宁宇,于业绍,杨季芳,吴友吕.文蛤病原菌(溶藻弧菌)的分离与性状及病文蛤组织的电镜观察[J].水产学报,1991,15(2):85~95
文蛤病原菌(溶藻弧菌)的分离与性状及病文蛤组织的电镜观察
CHARACTERISTICS OF VIBRIO ALGINOLYTICUS ISOLATED FROM DISEASED CLAM MERETRIX MERETRIX AND HISTOPATHOLOGICAL OBSERVATIONS ON DISEASED CLAM BY ELECTRON MICROSCOPE
投稿时间:1990-09-01  修订日期:1991-01-01
DOI:
中文关键词:   文蛤  弧菌病  溶藻弧菌  组织病理  电镜观察
英文关键词: clam  Meretrix meretrix  vibriosis  Vibrio alginolyticus  histopathology  electron microscopic observation
基金项目:
作者单位
郑国兴 东海水产研究所 上海 
李何 东海水产研究所 上海 
黄宁宇 东海水产研究所 上海 
于业绍 东海水产研究所 上海 
杨季芳 国家海洋局第二海洋研究所 杭州 
吴友吕 国家海洋局第二海洋研究所 杭州 
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中文摘要:
      近年来,江苏南部沿海在8~10月份常发生文蛤大批死亡,从病文蛤体内分离到病原菌,经人工感染试验得到证实。病原菌为革兰氏阴性短杆菌,以极生单鞭毛运动,在TCBS琼脂平板上形成黄色大菌落,在固体培养基上能游动。发酵葡萄糖产酸不产气,精氨酸—碱反应阴性,赖氨酸、鸟氨酸脱羧阳性。在无盐蛋白胨水中不能生长,在10%NaCl 蛋白胨水中生长良好。在43℃下能正常繁殖。对弧菌抑制剂0/129(150μg)和新生霉素(5μg)敏感,被鉴定为溶藻弧菌(Vibrio alginolyticus Sakazaki)。病文蛤超薄切片的电镜观察表明,肠上皮及肝组织被细菌侵袭。病原菌在肠上皮细胞质中增生,形成上百个细菌的集群。上皮细胞核变形,被挤向一侧,线粒体内嵴模糊,部分上皮细胞微绒毛的结构被严重破坏,细菌周围的组织被腐蚀成空斑。
英文摘要:
      An eplzootic of high mortality of the hard clam Meretrix meretrix occured along the seabeach of the East China Sea, art investigating had been car-ried out during the period of August to October 1988. The diseased clam looked verythin, it could not sink into muddy sand and its shells loosely closed. There was noresidual of food in the digestive tract. The body colour became to light red. Para-sitic copepoda were found in the mantle cavity. Pathogenic bacterium was isolated from the diseased clam. Infection experi-ments ascertained that the bacteria were the cause of the disease.The isolates showed Gram-negative short, rods with a single polar flagellum. All strains grew well andformed large yellow colonies on TCBS ager and swarmed strikingly on marine agar.They produced acid but no gas from glucose. Tests for the decarboxylation of lysineand ornithine were positive and arginine negative. They could net grow in peptonewater lacking NaCl but grew well in peptone water containing 10% NaCl. They couldnormally grow at 43℃. The organisms were sensitive to vibriostatic agent 0/129(150 μg) and novobiocin (5μg). Thus the pathogenic bacterium was identified as Vibrio alginolyticus. The bacteria grew in 1% peptone water at optimum temperaturesof 20-37C, at optimum NaCl of 1-8% and at optimum pH from 6 to 10 respectively. Electron microscope examinations of the histological ultrathin sections of the diseased clam demonstrated the presence of rod-shaped pathogenic bacteria in af-fected intestinal epithelial cells and liver cells.The pathogen multiplied and formedcolonies in the cytoplasm of intestinal epithelial cells. Nucleus degenerated and waspushed to the margin of the cells. Microvilli and cytochondriomes in the epithelialcells were destroyed. The digestive tube and liver tissues of the host become necro-sis and histolysis.
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