文章摘要
张四明,龙华,张兴忠.方正银鲫、白鲫与鲫线粒体DNA限制性内切酶酶切比较[J].水产学报,1992,16(2):120~129
方正银鲫、白鲫与鲫线粒体DNA限制性内切酶酶切比较
COMPARATIVE STUDY QN RESTRICTION ENDONUCLEASE DIGESTIUN OF mtDNAs FUR CARA55IUS AURATUS GIBELIC,CARAS5IU5 AURATUS CUYIERI AND CARA55IUS AURATUS AURATUS
投稿时间:1991-08-01  修订日期:1992-01-01
DOI:
中文关键词: 线粒体DNA  限制性内切酶  方正银鲫  白鲫  
英文关键词: mitochondrial DNA  restriction endonuclease  Caraasus auratus gibetio  Oarasszus auratrus cuvier  Carassiaus auratus aaaratu
基金项目:
作者单位
张四明 长江水产研究所 沙市 434000 
龙华 长江水产研究所 沙市 434000 
张兴忠 长江水产研究所 沙市 434000 
摘要点击次数: 1606
全文下载次数: 1230
中文摘要:
      用 Bam HI、Eco RI、Dra I、Hinf I、Hind III、Hpa II、Msp I、Pst I、Sau 3AI、Sma I、Xba I、Xho I和 Sal I等十三种限制性内切酶对方正银鲫(Carassius aur-atus gibelio)、白鲫(Carassius auratus cuvieri)和鲫(Carassius auratus auratus)的线粒体 DNA(mt DNA)进行单酶酶切以及其中八种识别六碱基对序列的限制性内切酶的双酶酶切,经琼脂糖凝胶电泳后,分析且计算出各酶切片断大小,得出三种鲫鱼的 mt DNA分子大小:方正银鲫为 15990±90碱基对(bp);白鲫为 16600±130碱基对(bP);鲫为 15540±140碱基对(bp),并且分别建立了方正银鲫、白鲫和鲫 mt DNA由 Bam HI、Pst I、Eco RI 及 Xba I等四种限制性内切酶构建的酶切图谱。
英文摘要:
      The mitochondrial DNAs (mtDNAs) of Carassius auratusbeZia,arassas auratus cuiera and Carassius auratus auratus Were digested by thirteenrestriction endonucleases, Via..Bam HI, Eco RI,13ra I,I,Hird III,Hpa TT,Msr I,Pst IAI, rna I, Xba I,I and rfaZ I. The double enzyme digestions wereconducted by using Bam HI, Eco RI, d III, Sma I, a I, SL I and Pst I. Thefragments Were obtained and their molecular sixes Were estimated. by aga.rase geIelectrophoresis. The size of mtDNAs for C. aecratusbeZin, C. auratus ceaiera and C.anratus auratusare 1599±90bp, 1600±130bp and 15540±140bp respectively.Taexperimental results demonstrated that the electrophoretic patterns of mtDNAsfor C. au-atusgibeaa, C. auratus cxiera and C. duratus aurdtus obtained by eithersingle or double enzyme digestion were significantly different, and the patternsof C. auraEusbebwv were obviously different among individuals by using Ha II.Besides, the restriction maps of mtDNAs from above species rere constructed byusing Bam HI, Pst I, Ecv RI and Xba I.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看