文章摘要
陈松林,刘宪亭,鲁大椿,章龙珍,傅朝君,方建平.家鱼冷冻精液激活、授精方法的研究[J].水产学报,1992,16(4):337~345
家鱼冷冻精液激活、授精方法的研究
THE ACTIVATION AND INSEMINATION METHODS OF CRYOPRESERVED SPERM IN CHINESE CARPS
投稿时间:1991-11-01  修订日期:1992-06-01
DOI:
中文关键词: 家鱼  冷冻精液  激活  授精  受精率
英文关键词: Chinese carps  cryopreserved sperm  activation  insemination  Fertility
基金项目:国家“七五”科技攻关课题
作者单位
陈松林 长江水产研究所 沙市 434000 
刘宪亭 长江水产研究所 沙市 434000 
鲁大椿 长江水产研究所 沙市 434000 
章龙珍 长江水产研究所 沙市 434000 
傅朝君 长江水产研究所 沙市 434000 
方建平 长江水产研究所 沙市 434000 
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中文摘要:
      激活—授精溶液渗透压对家鱼冻精的能育性有明显影响。当用1毫升冻精与4 毫升卵子授精时,冻精与鲜卵在水中与在 171和 342 mOsm/l NaCl 溶液中的受精率均很高(91%左右),无明显差别;当精卵量比为 1:16时,在水中的受精率明显低于在上述 NaCl 溶液中的受精率;当精卵量比升高到 1:32以上时,冻精在 174mOsm/l 溶液中的受精率明显高于其它二组。鲢卵在171和342 mOsm/l 溶液中维持受精能力的有效时间(6 分钟)明显长于在淡水中(2 分钟)。当精子稀释难的 pH 值偏碱(8.7)和冻精激活—授精溶液偏酸(pH6.3)时,冻精的受精率明显下降。解冻后精子在 4℃存放1.5小时活力和受精率下降不多,少数冻精可存活长达 16小时。冻精活力与受精率之间存在着明显正相关,相关系数为0.97,回归方程为 Y=3.68+1.23X。
英文摘要:
      The activation and insemination methuds of cryopreserved sperm from three species of Chinese carps (Ctenopharyngodon idella, Hypophthalmichthys molitrix and Megalobrama amblycephala) have been studied. The effect of the osmo-lality of activating solution an fertility of cryopreserved sperm was examined.When sperm-ova insemination ratio was 1:4(V/V),there was no significant di-fference in fertility of frozen sperm between in fresh water and. in 171-342mOsm/l solution. When sperm--ova ratio was 1:16,fertility of frozen sperm in NaCl solution with osmnlality of 171-342mOsm/l was significantly higher than that in fresh water. Ova could be fertilized for 6 min in above medium.but 2 min in fresh water.Basic extender (pH>8.7) was unsuitable for sperm storage,and the slightly acidic activating solution (pH6.3) rues unfavourable for the activation and insemination of cryopreserved sperm. The thawing sperm stored at 4℃ for 1.5 hour and did not significantly influence motility and fertility. A significant positive correlation (r=0.97) between the activity of cryopreserved sperm and fertility was shown. The regression equation was Y=3 .68 ± 1.23x.
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