文章摘要
许淑英,李焕林,邓国成,姜兰.草鱼出血病细胞培养弱毒疫苗的制备及其免疫效果[J].水产学报,1994,18(2):110~117
草鱼出血病细胞培养弱毒疫苗的制备及其免疫效果
THE PREPARATION AND IMMUNE EFFECT OF ATTENUATEDLIVE VACCINE OBTAINED THROUGH CELL CULTUREFOR HEMORRHAGE OF GRASS CARP
投稿时间:1993-08-13  修订日期:1993-01-01
DOI:
中文关键词: 草鱼出血病  细胞培养  弱毒疫苗  免疫效果
英文关键词: hemorrhage of grass carp  cell culture  attenuated live vaccine  immune offset
基金项目:
作者单位
许淑英 珠江水产研究所,广州 510380 
李焕林 珠江水产研究所,广州 510380 
邓国成 珠江水产研究所,广州 510380 
姜兰 珠江水产研究所,广州 510380 
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中文摘要:
      本文报道经筛选的GCHV-841毒株在草鱼吻端成纤维细胞系(PSF)中进行适应性培养,弱毒驯化,细胞培养弱毒疫苗的制备,安全性观察,不同稀释度和剂量的免疫保护率测定及保存试验.GCHV-841毒株在PSF细胞中继代驯化至53代达到减毒目的.筛选得53~59代作毒种制备的细胞弱毒疫苗使用安全.用稀释10~104倍的疫苗,0.1~0.3毫升/尾剂量注射草鱼,其免疫保护率均在85%以上.生产上使用疫苗的适宜浓度和剂量是稀释102倍和0.2毫升/尾.冻干疫苗宜保存于4℃以下.较合适的保存期是6个月(4℃)和18个月(-20℃).
英文摘要:
      This paper expounded that GCHV-841 sifted is cultured with adaptability in cell line(PSF) from the fibroblast cell of grass carp(Ctenopha-ryngoho ldella)snout tissue, attenuated domostication, preparation ofattenuated live vaccille obtailled through cell cultUre,observation on innoeuity,determination of percent relative protection with difforent dilutions and dosages,and test of storage.GCHV-841 has been domesticated continuously for 53 generations and has been attenuated. The attenuated live vaccine prepared with generation 53-59 is safe for use.The percent relativg protection of vaccine is above85%, when it was injected to grass carp with vaccine of diluted 10-104 tiniesand dosage 0. 1-0. 3ml/ind. The suitable concentration and dosage are diluted 102 times and 0. 2ml/ind. It is suitable to store the freeze-dried vaecine below 4℃.The comparatively suitable storage time is 6 months in 4℃ or 18 months in - 20℃.
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