文章摘要
宋平,李小迎,熊全沫.鲢鳙线粒体DNA的九种限制性内切酶酶场图谱的比较[J].水产学报,1994,18(3):221~230
鲢鳙线粒体DNA的九种限制性内切酶酶场图谱的比较
COMPARATIVE STUDY ON THE MAPS OF NINE ENDONUCLEASES OF mtDNA OF SILVER RESTRICTION CARP (HYPOPHTHALMICHTHYS MOLITRIX ) AND BIGHEAD (ARISTICHTHYS NOBILIS)
投稿时间:1993-07-05  修订日期:1992-12-01
DOI:
中文关键词: 链鱼  鳙鱼  mtDNA  限制性内切酶图谱
英文关键词: silver carp  bighead  mtDNA restriction physical map
基金项目:国家自然科学基金
作者单位
宋平 武汉大学生命科学学院, 30072 
李小迎 武汉大学生命科学学院, 30072 
熊全沫 武汉大学生命科学学院, 30072 
摘要点击次数: 1334
全文下载次数: 985
中文摘要:
      以十一种限制性内切酶对鲢鱼mtDNA进行单酶完全酶解的切点数:SalI和BglⅡ为0;PstI.XhoI.KpnI和SacI为1;HpaI和XbaI为2;BamHI为3;BglI和EcoRI为4.以九种限制性内切酶对鳙鱼mtDNA进行单酶完全酶解,其切点数;PstI.XhoI.SalI.KpnI.BglⅡ均为1;HPaI和XbaI为2;SacI为3;BglI为4.采用琼脂糖凝胶电泳法测得鲢鱼mtDNA分子量为15930±220碱基对(bp);鳙鱼mtDNA分子量为16650±150碱基对(bp).用单、双酶解法和部份酶解法构建了鲢鱼九种酶18个切点和鳙鱼九种酶16个切点的限制性内切酶酶切图谱.另外,对这两种鱼的酶解位点数,片段大小和限制酶图谱进行了比较.
英文摘要:
      he mitochondria) DNA(mtDNA)of silver carp were digested by 11 restriction endonucleases which were Bam I,Bgl I,Bgl B,EcoR I,Hpa I,Kpn I,Pst I, Sac I,Sal I,Xba I and Xho I .The fragments cleaved by these enzymes are 3,4,0,4,2,1, 1,1,0,2 and 1,respectively. The mtDNA of bighead were digested by restriction endonucleases Bgl I,Bgl B,Hpa I,Rpn I,Pst I,Sac I,Sal I,Xba I and Xho I into 4,1, 2,1,1,3,1,2 and 1 fragments,respectively. The sizes of mtDNA were estimated by agarose gel electrophoresis and they are 15930士220 by for silver carp and 16650士150bp for bighead. The maps of 9 restriction endonucleases for silver carp(18 cleaved sites)and bighead(16 cleaved sites) were constructed after digesting by the single,double or partial enzyme. The paper also compared the enzyme-digested sites,the size of fragment and the maps of restriction endonucleased between both fishes.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看