文章摘要
陈细法,吴定虎,黄槐,陈平.草虾感染草虾杆状病毒(MBV)后肝胰腺上皮细胞病理变化的电镜观察[J].水产学报,1994,18(4):265~271
草虾感染草虾杆状病毒(MBV)后肝胰腺上皮细胞病理变化的电镜观察
ELECTRON MICROSCOPIC OBSERVATION ON CYTOPATHOLOGIC CHANGES IN HEPATOPANCREA EPITHELLAL CELLS INFECTED BY MBV (PENAEUS MONODON LACULOVIRUS)
投稿时间:1993-11-11  修订日期:1993-01-01
DOI:
中文关键词: 草虾  草虾杆状病毒  细胞病理变化
英文关键词: shrimp  cytopathologic change  Penaeus monodon baculovirus
基金项目:
作者单位
陈细法 厦门大学, 361005 
吴定虎 厦门大学, 361005 
黄槐 厦门市水产研究所, 361005 
陈平 厦门大学, 361005 
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中文摘要:
      本文用改良的电镜固定液和新包埋剂制备超薄切片,在电镜下观察健康和感染MBV的肝胰腺上皮细胞的超微病理变化,结果发现感染细胞的核质中有1个或多个包涵体和大量病毒粒子.随着包涵体和MBV粒子增多,核染色质凝聚,核膜局部扩张,部份破裂,甚至完全消失,直至整个核崩解,使得MBV和包涵体游离出来.胞质水肿,内质网髓鞘样变性,滑面内质网增生,脂滴增多,线粒体绒团样变性和并发细菌混合感染.作者认为核内包涵体和病毒大量存在,并引起严重病变,可用于诊断草虾病毒病的特征性指标之一,而细菌混合感染是重要的辅助诊断指标之一.肝胰腺脂肪代谢障碍有可能影响病后草虾的正常生长.
英文摘要:
      The ultrathin sections manufactured by improved fixative for biological samples of electron microscope and new embedding media were used to study the ultrastructure changes of the normal and MBV-infected epithelial cells of the hepatopancreas under JEM-1200. The results indicated there was one or more intranuclear inclusion bodies and a great number of virions in the karyoplasm of the infected cells. As the number of inclusion bodies and virions increased,the unclear chromatins agglutinated and the nucleolar migrated,the nuclear membrane partially dilated,disrupted or even completely disappeared. At last,the whole nucleus would disintegrate and drift the inclusion bodies out of the nucleus. In this periods,the oedema and necrosis of cell plasms,the myeloid deterioration of endoplasmic reticulum,the hyperplasia of agranular endoplasmic reticulum and lipiddroplets,the down-wass-like deterioration of mitochondrias and the secondary bacterial mixed infections also be found in the studies. The authors consider that the large number of intranuclear inclusion bodies and MBV (existed in the karyopalsm),which caused the severe cytopathologic changes,could serve as one of the characteristic indication in diagnosing the viral disease of P. monodons. The bacterial mixed infection is one of the important auxiliary diagnostic indication. The lipoidosis and pathologic changes of mitochondrias in epithelial cells of the hepatopancrea may have effects on the normal growth of the surviving P. monodons.
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