文章摘要
刘向宇,陆仁后.卤虫无节幼体和日本绒螯蟹合浦亚种蚤状幼体的冷冻保存[J].水产学报,1994,18(4):321~325
卤虫无节幼体和日本绒螯蟹合浦亚种蚤状幼体的冷冻保存
CRYOPRESERVATION OF NAUPLII ARTEMIA SPP.AND ZOEA ERIOCHEIR JAPONICUS HEPUENSIS
投稿时间:1993-03-01  修订日期:1992-12-01
DOI:
中文关键词: 冷冻保存  冷冻保护剂  卤虫  日本绒螯蟹合浦亚种  无节幼体  蚤状幼体
英文关键词: cryopreservation  cryoprotective agent  Anemia spp  Eriocheir japonicus hepuensis  nauplii  zoea
基金项目:
作者单位
刘向宇 中国科学院水生生物研究所, 武汉 430072 
陆仁后 中国科学院水生生物研究所, 武汉 430072 
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中文摘要:
      本文研究了葡萄糖、蔗糖和甘油三种冷冻保护剂在不同浓度下对卤虫无节幼体的冷冻保护作用.研究表明用20%甘油进行保护,无节幼体在-30℃下耐受9-10分钟,存活率高达86.4%,对照组冷冻至-30℃则全部死亡.本文还研究了浓度为20%的甘油、DMSO和甲醇对日本绒螯蟹合浦亚种 状幼体的冷冻保护作用,结果表明用20%甲醇保护的蚤状幼体当冷冻至-24℃时存活率仍有75.3%.
英文摘要:
      Chill tolerance of nauplii of Anemia spp. put into freezer at -30℃ for 30 minutes with and without the addition of glucose, sucrose and glycerol at various concentrations was determined. The average liquid cooling rate was 3-5℃ /min, and the average solid cooling rate was 1-1. 3℃/min. All of the control without the addition of the cryoprotective agent died, the addition of the cryoprotective agent enhances the cooling tolerance of nauplii, and the survival varied according to the kind of cryoprotective agent,concentration and balance time, the highest survival of nauplii reached 86. 4% in 20% glycerol when frozen to -30℃for 9-10 minutes. Chill tolerance of zoea of Eriocheir japonicus hepuensis at -25℃was also studied with 20% glycerol,DMSO and methanol. The average liquid cooling rate was 3-4℃ /min, the average solid cooling rate was 0. 3-0. 5℃ /min. All of the control died when frozen to -23℃, the best rsult was 75. 3% survived in 20% methanol when frozen to -24℃. Besides, the significance of these results for the development of cryogenic larval banking techniques was discussed.
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