文章摘要
王素娟.羊栖菜Sargasum_fusiforme原生质体的分离和培养[J].水产学报,1996,20(3):
羊栖菜Sargasum_fusiforme原生质体的分离和培养
THE ISOLATION AND CULTURE OF PROTOPLASTS FROM SARGASSUM FUSIFORME ( PHAEOPHYTA, SARGASSACEAE)
投稿时间:2014-05-11  修订日期:2014-05-11
DOI:
中文关键词: 羊栖菜  原生质体  分离  培养
英文关键词: Sargassum fusiforme, Protoplast , Isolation, Culture
基金项目:
作者单位地址
王素娟 上海水产大学 上海水产大学
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中文摘要:
      本研究多次获得原生质体,明确了酶解原生质体的内在因素与外在条件,诸如酶溶剂的种类及浓度,酶及组成,酶解温度及pH,采种藻时间及取用部位。分离的原生质体经初步培养,发现少数2分裂,最好的有成6分裂的细胞团,但尚未成株。研究结果为扩大培养打下了良好的基础。此外,还报道了用海洋细菌酶解的试验及意义 。
英文摘要:
      Proto pla st s f r om one species of br ow n alga Sargassum f usif orme ( Harv. ) Setchell had been obta ined w ith the applicat ion o f wa ll degrading enzymes. The inte rnal facto rs and external co ndit ions o f the iso latio n o f proto pla sts , such a s kinds and co nce nt rat io ns of o smo regulate d re agent, kinds and combinat ions of enzymes, isola ted tempe rature a nd pH, t ime o f ma ter ial collect ions and part of thallus, are def inited now . Hav ing been cultur ed for 3 da ys, some pr otoplast s regenerated cel-l w alls and div ided into tw o cel ls. Six-cell cal lus-like cel-l ma ss wa s also obtained af te r being cultur ed for 6 day s. The se re sult s are g oo d basis f or further culture in large scale. The applica tio n o f marine bacteria on isolat ing vegetat ive cel ls o f these alg a is reported in this pa per.
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