文章摘要
李美同.土霉素在鳗鲡组织中残留的消除规律[J].水产学报,1997,21(1):
土霉素在鳗鲡组织中残留的消除规律
STUDIES ON THE DEPLETION OF OX YTETRACYCLINE RESIDUES ON EEL( ANGUILLA JAPONICA) TISSUES
投稿时间:2014-05-10  修订日期:2014-05-10
DOI:
中文关键词: 鳗鲡  土霉素  残留  消除规律
英文关键词: Anguilla jap onica, Oxytet racycline, Residue, Depletion
基金项目:
作者单位地址
李美同 中国兽药监察所 中国兽药监察所
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中文摘要:
      在23~ 27e 水温中以每天200mg / kg b. w. 剂量灌服土霉素五天, 测定鳗鲡组织中 土霉素残留的消除规律。停药后每天剖取五条鱼的肉和肝, 用4%三氯乙酸- 0. 02mol/ L 磷酸氢二 钠水溶液和三氯甲烷匀浆, 用石油醚除去脂肪。水层用固相萃取C18柱净化后, 用HPLC 法在 360nm 下测定土霉素残留。色谱柱: ODS- 5 L 250 @ 4. 0mm。流动相: 甲醇+ 乙腈 二甲基甲酰胺 EDTA- 柠檬酸钠缓冲液= 1 1. 2 0. 42 1. 9。停药1 天鱼肉中土霉素含量达到1. 0mg/ kg, 停 药7 天降低至0. 1mg/ kg 。
英文摘要:
      Eels were kept in experimental tanks w ith aerated tapw ater at temperatures of 23~ 27 e . T he weig ht per fish w as 200~ 300g. The eels w ere fed medicated feed by syringe. T he dosage of Ox ytet racycline ( OTC) w as 200mg/ kg b. w . per day for 5 days. Muscle and liver tissues were taken from five eels at hours 0, 6, 12, 24, 48, 72, 120, 168, 216 af ter the last dose w ith OTC. Tissues samples w ere homogenized w ith 4% t richloroacet ic acid - 0. 02mol/ LNa2HPO4 aqueous solut ion and trichloromethane, fat w as removed by pet roleum ether. The aqueous layer was purified by a C18 solid-phase ext ract ion column. The OTC residues w ere determined by HPLC external standard method using a SGE- ODS- 5 L 250 @ 4. 0mmID column w ith a mobile phase of methano-l acetonit rile-DMF-buffer ( Na2EDTA-citric acid-sodium cit ratew ater) = 1B1. 2B0. 42B1. 9 at UV detect ionwavelength of 360nm. The OT C level in eel muscle reached 1. 0mg/ kg at 24 hours and decreased to 0. 1mg / kg at 168 hours ( 7 days) after w ithdrawal.
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