文章摘要
岳玉环.鳗鲡冠状病毒样病毒的细胞分离与培养[J].水产学报,1998,22(3):
鳗鲡冠状病毒样病毒的细胞分离与培养
CELL ISOLATION AND CULTUREOF ANGUILLA ANGUILLA CORONAVIRUS-LIKE VIRUS
投稿时间:2014-05-06  修订日期:2014-05-06
DOI:
中文关键词: 欧洲鳗鲡 冠状病毒 样病毒 细胞培养
英文关键词: Anguilla anguilla ,Coronavirus-like virus , Cell culture
基金项目:
作者单位地址
岳玉环 解放军农牧大学水产动物疾病研究中心 解放军农牧大学水产动物疾病研究中心
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中文摘要:
      实验选用4种鱼类传代细胞,对首次发现的鳗鲡“狂游病”的病原鳗鲡冠状病毒样病毒进行了细胞分离与培养。本研究在鲤上皮乳头状瘤细胞(EPC)中复制了鳗鲡冠状病毒样病毒粒子,并经病毒细胞培养物的电镜负染和超薄切片法检查得到了证实。
英文摘要:
      I n this exper iment , 4 of the fish cell l ines were selected to isolate and culture Eel Coronavirus-like Virus that was a pathogen of eel(Angui lla anguil la)“madswim- disease” .The r esults showed that the Eel Coronavir us-l ike Virus could be repl icated in epithel ioma papillosum cypr ini (EPC).The growth of the virus in EPC was conf irmed by electron microscopy method both in negat ive-staining and ultr athin sect ion specimens f rom observa tion of theviral cell cul tur es .And i t was also ver ified tentat ively that ra inbow trout liver cells(R1), grass carp kidney cells (GCK)and grass carp oavry cells(CO)were not sensi tive to this virus .
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