文章摘要
杨蕙萍.皱纹盘鲍淀粉酶和褐藻酸酶的研究[J].水产学报,1998,22(4):
皱纹盘鲍淀粉酶和褐藻酸酶的研究
THE PROPERTIES OF AMYLASE AND ALGALASE IN HALIOTIS DISCUS HANNAI
投稿时间:2014-05-06  修订日期:2014-05-06
DOI:
中文关键词: 皱纹盘鲍 淀粉酶 褐藻酸酶 金属离子
英文关键词: Haliotis discus hannai , Amylase , Algalase , Metal ions
基金项目:
作者单位地址
杨蕙萍 中国科学院海洋研究所 中国科学院海洋研究所
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中文摘要:
      利用分光光度计比色法测定了2~3cm的皱纹盘鲍(HaliotisdiscushannaiIno)的淀粉酶和褐藻酸酶的最适温度和最适pH以及11种金属离子对其的影响。结果表明:最适温度分别为30℃、35℃,最适pH分别为6.24、7.19,在最适温度下的活化能分别为5.03×104J/mol和1.33×104J/mol;皱纹盘鲍的三种主要消化酶的活性依次为:褐藻酸酶>蛋白酶>淀粉酶。Cu2+、Hg2+、Ag+、Pb2+对淀粉酶活性具有显著的抑制作用,其他离子则具有促进作用,其中又以Mn2+、Ba2+、Ca2+三种金属离子尤为突出,其促进作用比对照组高一倍以上。而对褐藻酸酶,Zn2+、Ag+、Hg2+、Li2+、Ba2+具显著的抑制作用,其他离子则没有作用。
英文摘要:
      The amylase and algalase of the Pacif ic abalone (Haliotis discus hannai) wer e measured during the length of 2 -3 cm by means of spect rophotome ter .The optimum tempe ratures f or amylase and algalase were 30 ℃ and 35 ℃, The opt imum pH values were 6 .24 and 7 .19 .Under the opt imum temper ature the act ive energy of two enzymes were 5 . 03 ×104 J/mol and 1 .33 ×104 J/mol r espect ively ;the act ivi ty of amylase and algalase were detected when eleven metal ions existed .The resul ts indicated that Cu2 + 、Hg2 、Ag 、Pb2 significantly inhibited the act ivi ty of amylase , and other metal ions activated the activity of amylase .Mn2 , Ba2 and Ca2 can act iva te the amylase two t imes of that in cont rol group .For the algalase , Zn2 , Ag , Hg2 , Li2 and Ba2 significant ly inhibited its activi ty , and the other metal ions had no effects on the algalase .
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