文章摘要
陆宏达.中华绒螯蟹小核糖核酸病毒病及其组织病理学[J].水产学报,1999,23(1):
中华绒螯蟹小核糖核酸病毒病及其组织病理学
A PICORNAVIRUS DISEASE AND HISTOPATHOLOGY OF ERIOCHEIR SINENSIS LU Hong-Da
投稿时间:2014-04-18  修订日期:2014-04-18
DOI:
中文关键词: 中华绒螯蟹 组织病理学 RNA病毒病
英文关键词: Eriocheir sinensis, Picornavirus, Histopathology
基金项目:
作者单位地址
陆宏达 上海水产大学渔业学院 上海水产大学渔业学院
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中文摘要:
      本文对所谓“抖抖病”的中华绒螯蟹病蟹病因进行了分析研究。经电镜观察,病蟹的心脏、腹神经节、鳃、肠和肝胰腺组织中存在着球状病毒粒子,病毒无囊膜,直径为28~32nm左右,分布在细胞浆内,不形成包涵体。从病毒形态和染色反应特性确定该病毒为小RNA病毒科(Picornaviridae)病毒。经人工注射病蟹组织浆除菌上清液,被感染的健康蟹出现与自然发病蟹步足严重抖动等完全一样的症状,表明病蟹中的小RNA病毒为该病病原。病蟹病理特征为:细胞肿大,核溶解或固缩,线粒体肿大、嵴断裂、扭曲或坏死溶解。病变最严重的区域,组织细胞坏死崩解成一片无结构的物质。 更多还原
英文摘要:
      T he cause of Eriocheir sinensis disease, w hich w as called as .. Trembling Disease.. by the f ish farmers, w as studied. Ex aminat ion by electron microscopy show ed that there w ere a numbers of v iral part icles in the t issues of heart、abdominal nerve ganglion、g ill、intestine and hepatopancreas and there was no envelope outside v irion. T he diameter of virion was approx inately from 28nm to 30nm. Virions occurred in the cy toplasm and did not form the inclusion body. According to the characters of viral morphology and dyeability, the virus belonged to Picornaviridae. The healthy Eriocheir sinensis after injected w ith f iltered fluid from ground tissues of diseased Eriocheir sinensis appeared the same symptom of legs t rembling as that of the natural diseased one. This result conf irmed that the picornavirus was pathogen of the disease. The histopathological changes: cells oedema, karyolysis or karyopyknosis, mitochondria oedema, mitochondria cristae broken、curved and necrolysis. The necrotic cells became the substances of no cell st ructure in the severe necrot ic areas.
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