文章摘要
杨爱国.6_二甲基氨基嘌呤诱导栉孔扇贝三倍体[J].水产学报,1999,23(3):
6_二甲基氨基嘌呤诱导栉孔扇贝三倍体
TRIPLOID INDUCTION IN CHLAMYS FARRERI BY APPLICATION OF 6 -DIMTHYLAMINOPURINE
投稿时间:2014-04-18  修订日期:2014-04-18
DOI:
中文关键词: 栉孔扇贝 二甲基氨基嘌呤 三倍体诱导
英文关键词: Chlamys farreri , 6 -Dimethylaminopurine(6 -DMAP), Triploid induce
基金项目:国家海洋863 计划资助
作者单位地址
杨爱国 中国水产科学研究院黄海水产研究所 中国水产科学研究院黄海水产研究所
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中文摘要:
      用6 - 二甲基氨基嘌呤(6 - DMAP) 处理,对抑制栉孔扇贝第二极体释放产生三倍体的适宜诱导参数进行了试验研究,结合DAPI染色和荧光显微镜观察,分析了开始处理前和处理结束时的受精卵染色体核相组成与三倍体诱导率和幼虫成活率的关系,对三倍体处理组和对照组幼虫的生长进行了观察。结果表明,获得充分成熟的卵子、起始处理时间以第一极体占40 % 、药物浓度60 mgL、持续处理时间15 min 及产卵至处理结束保持水温20 ℃,三倍体诱导率可稳定在80 %以上,孵化率达70 % 左右。
英文摘要:
      To produce tripoids , fertilized eggs of Chlamys(Azumapecten)farreri were treated with 6 -dimethylaminopurine (6 -DMAP)to inhibit the extrusion of the second polar body .Using dihydrochloride (DAPI ) stain and fluorescence microscopic observation , the relation between chromosome karyotype and triploid induction and larval survival percentage , the effects of egg maturation and spawning temperature on the synchronous development of fertilized eggs were analyzed . It was concluded that the optimism induction parameters for farming industry are as following : completely mature eggs , initial treatment time when the first polar body occupied 40 %, 60mg L of drug concentration , 15 min of treatment duration and water temperature kept at 20 ℃ from spawning to the end of treatment .Under the conditions , triploids can be obtained with an induction rate higher than 80 % and an hatching rate up to 70 %.
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