文章摘要
李思发.中国大陆沿海六水系绒螯蟹_中华绒_省略__群体亲缘关系_RAPD指纹标记[J].水产学报,1999,23(4):
中国大陆沿海六水系绒螯蟹_中华绒_省略__群体亲缘关系_RAPD指纹标记
PHYLOGENESIS OF POPULATIONS OF MITTEN CRABS ( EROCHEIR SINENSIS, E. JAPONICUS) IN SIX RIVER SYSTEMS OF MAINLAND CHINA: RAPD FINGERPRINTING MARKER
投稿时间:2014-04-18  修订日期:2014-04-18
DOI:
中文关键词: 中华绒螯蟹 日本绒螯蟹 群体 亲缘关系 RAPD技术
英文关键词: Eriocheir sinensis, Eriocheir japonicus , Population, Phylogenesis, RAPD Fingerprinting marker
基金项目:国家/ 九五0 科技攻关项目( 中华绒螯蟹种质鉴定技术研究) , 96- 008- 01- 03- 05 号。
作者单位地址
李思发 农业部水产增养殖生态、生理重点开放实验室 农业部水产增养殖生态、生理重点开放实验室
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中文摘要:
      用 4 8个具有丰富多态性的 10碱基随机引物对辽河、黄河、长江、瓯江、珠江和南流江的中华绒螯蟹和日本绒螯蟹的 6个群体进行RAPD分析。结果发现 :(1)两个引物具有群体特异性带 ,其中Z2扩增的 880bp片段为珠江蟹和南流江蟹群体中所特有 ,而 70 0bp片段为长江蟹、辽河蟹、黄河蟹和瓯江蟹所特有 ,可作为区别日本绒螯蟹和中华绒螯蟹的分子遗传标记 ;(2 )Opp17扩增的 94 7bp片段的出现频率 ,在长江、黄河和辽河群体显著地从南到北递减 ,长江蟹 87.50 %、黄河蟹4 1.66%、辽河蟹 10 .83%。这一遗传渐变的度量可作为区别这三个水系的中华绒螯蟹种群的判据 ;(3) 6水系群体内的平均遗传相似度依次为 :辽河蟹 0 .90 8>南流江蟹 0 .897>瓯江蟹和珠江蟹 0 .895>黄河蟹 0 .890 >长江蟹 0 .850 ;6群体间遗传距离及其UPGMA、NJ聚类分析进一步表明 ,南流江蟹、珠江蟹同长江蟹、辽河蟹及黄河蟹在基因组之间存在明显的歧化。 更多还原
英文摘要:
      RAPD was employed to assess the genet ic variations among 6 Eriocheir sinensis and Eriocheir japonicus populat ions distributed in Liaohe, Yellow , Yangtze, Oujiang, Pearl and Nanliu River systems. ( 1) Two primers could amplify distinct ive bands in 48 polymorphic 10- mer primers. The 880bp band amplified by primer Z2 was characterized only by Pearl and Nanliu River populations, and the 700bp band was characterized only by Yangt ze, Yellow, Liaohe and Oujiang River populations, both bands can be used as fingerprint ing marker to identify E. sinensis and E. japonicus . (2) The frequency of 947bp band amplified by primer Opp17 declined from south to north, e. g. , Yangtze River population ( 87. 50%) , Yellow River population ( 41. 66% ) and Liaohe River population ( 10. 83% ) . ( 3) The average genetic similarity index within each population ranked as Liaohe River 0. 908> Nanliu River 0 . 897 > Oujiang River and Pearl River 0. 895 > Yellow River 0. 890> Yantze River 0. 850. The genetic distances and their UPGMA and NJ dendrogram of six populations indicated that there was an obvious divergence between Pearl River, Nanliu River populations in southern China and Liaohe River, Yellow River and Yangtze River populations in northern China.
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