文章摘要
孙修涛.中国对虾卵水的特性和精子的应答[J].水产学报,2000,24(1):
中国对虾卵水的特性和精子的应答
The property of egg..water and the response of sperm of Penaeus chinensis
投稿时间:2014-04-17  修订日期:2014-04-17
DOI:
中文关键词: 中国对虾 卵水 精子 应答 激活 时间特性
英文关键词: Penaeus chinensis , egg..water, sperm, respons, activation
基金项目:国家攀登计划B 资助项目( 海养生物优抗研究专题对虾受精过程和胚胎发育的生物学研究) ,PDB- 6- 2- 3 号。
作者单位地址
孙修涛 中国水产科学研究院黄海水产研究所 中国水产科学研究院黄海水产研究所
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中文摘要:
      研究了中国对虾卵水的采集、保存方法、紫外吸收特性和有效期等方面的内容, 并应用卵水研 究了交尾期雄虾、雌虾及产卵期雌虾精荚或纳精囊中精子对卵水的应答开始时间、必要的反应时间等响应卵 水的时间特性以及精子的存活期等。结果表明, 卵水经液氮保存或先经液氮后转入普通冰柜保持冻结状态7 个月后诱导精子激活效力无显著差异。精子在自然温度( 10 .. ) 普通海水中可在10h 内保持对卵水的响应能 力。交尾期精子最初响应卵水有5~ 10min 的迟延, 而产卵期精子延迟很短。
英文摘要:
      The shrimp, Penaeus chinensis used in this test was bought f rom Yellow Sea in March, 1995, for the collection of egg..water EW, as well as the autumn shrimp was bought in October of the same year. The shrimp was raised in marine laboratory and fed with Polychaeta and shellfish, the sand filtered sea water was used in the experiment . The egg..water EW was collected and the characters were examined and reported as well. The results were composed of the collection, storing, life..span of the egg..water, and the response of sperm isolated f rom different parts of male reproductive system and mated female. The results are ordered as follows. 1) The activating ability could be kept for 7 months or more as stored in liquid nitrogen or stored in refrigerator ( - 20 .. ) after inflicted in liquid N2( - 196 .. ) ; and it would lose the ability in normal temperature after 8..10 hours. It is a useful character on sperm quality checking in production. 2) The parallel examination indicates that EW is the only eff icient inductor to activate sperm, compared with sea water, ovary wash liquid, ovary isolated liquid and distilled water. 3) The ultraviolet absorbancy of EW has high peak on 213. 2 nm and a little peak on 268. 0 nm. 4) The initial interval of sperm isolated from mated female responding to EW was 5..8 minutes in autumn but immediate in spring. 5) The activating rates of sperm induced by EW varied in 30%..80% in autumn, and superior to 90% in spring. There has been a 7%..13% space act ivation induced by sea water without EW. 6) The life..span of sperm isolated from thylcum of autumn mated female was 10- 10. 5 hours limited under natural temperature( 9. 5 .. ) bathed in sea water, and 6. 0- 8. 5 hours in 17. 0 .. 1 .. . The life..span of sperm had no significance in male and female in autumn.
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