文章摘要
江世贵.中华乌塘鳢精子的生物学特性及其超低温保存[J].水产学报,2000,24(2):
中华乌塘鳢精子的生物学特性及其超低温保存
The biological characteristics of Bostrichthys sinensis spermatozoa and its cryopreservation
投稿时间:2014-04-17  修订日期:2014-04-17
DOI:
中文关键词: 中华乌塘鳢 精子 激活 超低温保存
英文关键词: Bostrichthys sinensis, spermatozoa, activation, cryopreservation
基金项目:
作者单位地址
江世贵 中国水产科学研究院南海水产研究所 中国水产科学研究院南海水产研究所
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中文摘要:
      对中华乌塘鳢精子激活的生理生态学进行了研究, 探讨了中华乌塘鳢精子超低温保存方法。 中华乌塘鳢精子激活的适宜盐度为5~ 15; 其精子的激活不仅与激活溶液盐度有关, 而且与激活溶液的离子 成份有关, 激活溶液中K+ 的存在在一定程度上对精子的活动有抑制作用; 中华乌塘鳢精子对pH 的适应性 强, 在pH 5. 5~ 9. 5 范围内, 激活率均在70%以上, 尤以中性及弱酸性条件下的激活率最高, 在pH 6. 0 时精子 活力最好。使用筛选出的精子保存液, 以二甲亚砜( DMSO) 为抗冻保护剂, 采用快速冷冻和解冻方法, 成功地 进行中华乌塘鳢精子超低温保存, 解冻后的中华乌塘鳢精子激活率达90%以上, 受精率达80%以上。
英文摘要:
      Effect of environmental factors on the activation of spermatozoa of Chinese ocellated sleeper, Bostrichthys sinensis ( Lac.p .de) , was studied. The optimum activat ing salinity for the spermatozoa was 5- 15. The activation of the spermatozoa concerned with not only osmosis or salinity, but also with composition of solution. The function of K+ was represented as a restraining factor to activation and movement of the spermatozoa in the present condition. The spermatozoa had a strong adaptability to the variation of pH, and the activating rates were more than 70% while pH of the activating solution varied from 5. 5 to 9. 5. The optimum pH of the activating solution was neutral or weak acidity for the spermatozoa, the swimming time of the spermatozoa was the longest when pH was 6. 0. The spermatozoa of Chinese ocellated sleeper were cryopreserved successfully by using a simple procedure . Sea water with salinity 40 or a solution containing 1. 5% NaCl , 1 . 5% KCl , 0. 25% CaCl2 and 0. 25% MgCl2, was practical extender for cryopreservation of Chinese ocellated sleeper spermatozoa. DMSO was an effective cryoprotectant, and its optimum concentration was 10% in this study. The activation rates of the pos-t thaw spermatozoa were almost as good as the control. s , namely fresh spermatozoa, and their fertilizat ion rates were over 80%, approaching the control. s.
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