文章摘要
李新辉.鳜鱼病毒核酸的初步分析[J].水产学报,2000,24(2):
鳜鱼病毒核酸的初步分析
Primary analysis for nucleic acid of Siniperca chuatsi virus
投稿时间:2014-04-17  修订日期:2014-04-17
DOI:
中文关键词: 鳜鱼病毒 核酸 克隆 序列 检测
英文关键词: Siniperca chuatsi , virus, nuleic acid, clone, sequence
基金项目:农业部和广东省科委以及广东省自然科学基金资助( 鳜鱼病毒核酸随机表达库建立及抗原基因筛选与表达研究) , 980907 号。
作者单位地址
李新辉 中国水产科学研究院珠江水产研究所 中国水产科学研究院珠江水产研究所
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中文摘要:
      提取鳜鱼病毒核酸,分别用DNase、RNase和绿豆芽核酸酸处理表明,该病毒为双链DNA病毒,用带EcoRⅠ酶切位点的随机引物扩增病毒核酸,获得扩增核酸带谱。经低熔点琼脂糖回收PCR产物,与质粒pUC19连接,获得三个重组子,已经对两个小插入片断进行了序列分析,插入序列分别为369bp和450bp。GenBank检测显示,尚未有类似的序列报导。
英文摘要:
      The virus nucleic acid was isolated from disease Siniperca chuatsi , and extracted with RNase, DNase and Mung bean nuclease respectively. The tests indicated that the virus genome is dsDNA. Part of the genome of Siniperca chuatsi virus were successful amplif ied by random primer ( contained the enzyme position of EcoR .. ) . The PCR products were recovered from low melting-temperature agarose and cloned in pUC19 plasmid. Three kinds of recombinant plasmids have been identified with EcoR ... Two kinds of it have been sequenced. There were 369bp and 450bp in size. It was demonstrated that there were not homology sequences against tho se in GenBank.
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