文章摘要
莫照兰.虾池有机污染物降解细菌的筛选[J].水产学报,2000,24(4):
虾池有机污染物降解细菌的筛选
Selection of organic-pollutants-degrading bacteria in shrimp ponds
投稿时间:2014-04-16  修订日期:2014-04-16
DOI:
中文关键词: 有机污染物降解细菌 饵料培养基 虾池 淤泥
英文关键词: organic-pollutants-degrading bacteria  shrimp-feed-medium  BOD  extra-enzyme  bacterial identification
基金项目:国家.. 九五.. 科技攻关资助项目(96- 005- 03- 01- 03)
作者单位地址
莫照兰 青岛海洋大学海洋生命学院 青岛海洋大学海洋生命学院
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中文摘要:
      在实验室条件下对612株海洋细菌和10株海水驯化淡水菌进行筛选,检测其快速降解水体中富营养有机物的能力。利用对虾饵料培养基、BOD仪、胞外酶检测等方法进行筛选,最后筛选到10株对富营养有机物具有 较高降解性能的细菌。所有的细菌均能产生明胶酶和脂酶(Tween-80),其中9株细菌能产生淀粉酶,8株产生卵磷脂酶,2株产生酪蛋白酶,1株产生褐藻胶酶。通过测量BOD来衡量10株细菌利用对虾饵料的效果,2天时能消化46.6%-59.5%的对虾饵料,5天时能消化50.8%-70.2%的对虾饵料。用常规生理生化方法将细菌鉴定到属,其中3株为弧菌属细菌(Vibrio spp.),3株为假单胞菌属细菌(Pseudomonas spp.),2株为发光杆菌属细菌(Photobacterium spp.),1株为气单胞菌属细菌(Aeromonas spp.),1株为枯草杆菌(Bacillus subtilis)。
英文摘要:
      612 bacterial strains from shrimp ponds and 10 freshwater strains which had been transplanted on the 2216E marine agar repeatedly were checked for their degrading abilities to the organic pollutants. 10 strains were selected by evaluating the growth on the shrimp-feed-medium, BOD in the liquid media and their extroenzymes activities. All these 10 strains were able to produce gelat inase, lipase ( Tween - 80) and 8 of these strains produced lecithinase, 9 produced amylase, 2 produced casinase, 1 produced alginase. All of them were able to degrade the feed of shrimp from 46. 5% to 59. 5% in two days and from 50. 8% to 72. 2% in five days. By the traditional physical and chemical methods, 3 of these strains were identified as Vibrio spp. , 3 as Pseudomonas spp. , 2 as Photobacterium spp. , 1 as Aeromonas spp. , and 1 as Bacillus subtilis.
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