文章摘要
郭琼林.草鱼_中华鳖淋巴细胞扫描电镜结构_省略_绵羊红细胞受体_CD_2_的检测[J].水产学报,2000,24(4):
草鱼_中华鳖淋巴细胞扫描电镜结构_省略_绵羊红细胞受体_CD_2_的检测
Observation of surface microstructure and investigation of SRBC receptor ( CD2) on lymphocyte of Ctenopharyngodon idellus and Trionyx sinensis
投稿时间:2014-04-16  修订日期:2014-04-16
DOI:
中文关键词: 草鱼 中华鳖 淋巴细胞异质性 免疫功能 CD2
英文关键词: Ctenopharyngodon idellus  Trionyx sinensis  heterogeneity of lymphocyte  E - rosette test  SRBC receptor( CD2)
基金项目:国家自然科学基金资助项目(39470555 和39670578) ; 湖北省自然科学基金以及中国科学院所长择优基金资助项目
作者单位地址
郭琼林 中国科学院水生生物研究所 中国科学院水生生物研究所
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中文摘要:
      草鱼淋巴细胞表面扫描电镜结构特征为有孔穴且较为光滑、具短小细锥状突起;中华鳖淋巴细胞、胸腺细胞表面扫描电镜结构特征为凹凸不平、较为光滑;它们均以表面凹凸不平特征的占大多数。E花环试验的光镜和扫描电镜观察显示:草鱼淋巴细胞E花环形成不明显,而中华鳖淋巴细胞、胸腺细胞的成花率分别为25%-34%和36%-47%,能形成花环的淋巴细胞、胸腺细胞以表面结构特征凹凸不平(类似哺乳动物T淋巴细胞)的为主。草鱼、中华鳖血液淋巴细胞和中华鳖胸腺细胞与抗人CD2单克隆抗体交叉反应的免疫组化检测显示中华鳖血液淋巴细胞和胸腺细胞的CD2阳性率分别为24%和33%。草鱼血液淋巴细胞CD2阳性反应不明显。
英文摘要:
      Under scanning electron microscope ( SEM ) , the surface microstructure on lymphocytes of Ctenopharyngodon idellus is characterized with full of bumpiness and holes, or rather smooth or protruding with small cones, whereas the surface microstructure on lymphocytes and thymocytes of Trionyx sinensis is characterized w ith full of bumpiness and holes, or rather smooth. The lymphocytes and thymocytes f illed with bumpiness and holes are evidently more than others. Under optic and scanning electron microscope, the rosette format ion of lymphocyte of C. idellus with SRBC is not obvious, whereas rosette forming percents in lymphocytes and thymocytes of T. sinensis are respectively 25% - 34% and 36%- 47% . The lymphocytes and thymocytes which form rosettes w ith SRBC are predominantly ones f illed with bumpiness and holes ( similar to T lymphocyte of mammalian) . The immuno-l histochemistry on the cross-reactivity by using monoclonal antibody against human CD2 shows that the CD2-positive percent of lymphocytes in peripheral blood and thymocytes of T. sinensis are respect ively 24% and 33% , but no cross-react ivity is found on lymphocytes in peripheral blood of C . idellus.
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