文章摘要
许玉德.杂交一代_尼罗罗非鱼_奥利亚罗非鱼_及其亲本基因组DNA的比较[J].水产学报,2001,25(1):
杂交一代_尼罗罗非鱼_奥利亚罗非鱼_及其亲本基因组DNA的比较
Oreochromis niloticus Comparison of genomic DNA of hybrid (♀× O. aureaus ♂) and its parents
投稿时间:2014-04-16  修订日期:2014-04-16
DOI:
中文关键词: 尼罗罗非鱼 奥利亚罗非鱼 杂交一代 精巢基因组DNA RAPD 杂种优势
英文关键词: Oreochromis niloticus  Oreochromis aureaus  hybrid  genomic DNA  Random Amplified Polymorphic DNA (RAPD)
基金项目:福建省水产厅资助(闽水外经9664)
作者单位地址
许玉德 厦门大学生物学系!福建厦门361005 福建省水产研究所!福建厦门361016 厦门大学生物学系!福建厦门361005; 福建省水产研究所!福建厦门361016
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中文摘要:
      采用RAPD技术,用20个随机引物对尼罗罗非鱼,奥利亚罗非鱼及其杂交一代的精巢DNA进行扩增反应,发现引物OPZ-14和OPZ-18在尼罗罗非鱼和奥利亚鱼之间扩增出有差异的DNA片段,作为鉴定两种鱼的分子标记有较高可信度,对这两种鱼的杂交一代基因组DNA的RAPD扩增能获得中够的多态性,20个引物中有8个引物扩增出差异性产物,表明杂交一代基因杂合性增强,这是杂种优势得以形成的重要遗传物质基础之一。
英文摘要:
      Using Random Amplified Polymorphic DNA (RAPD ) technique with 20 kinds of random primers , genomic DNA of hybrid ( Oreochromis niloticus ♀×Oreochromis aureaus ♂) and its parents were analysed. The resultsshowedthatonlywiththetwokindsofprimers,OPZ -14 andOPZ -18 ,species2specific fragments were produced between parent fishes , even between different fish belonging to the same species , and some of them could be used as molecular markers to identify O. niloticus and O. aureaus. And 8 primers generate polymorphic products among hybrid fish and its parents. DNA fragments amplified from the hybrid fish were more abundant than those from its parents , which may be the result of more abundant genes in the hybrid fish , because of heredity or mutation. These results provide some molecular biological basis for species identification and heterosis effect in fish.
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