文章摘要
宫相忠.球等鞭金藻8701的耐低温机理[J].水产学报,2001,25(1):
球等鞭金藻8701的耐低温机理
Mechanism of low temperature Isochrysis galbana Parke 8701 tolerance of Isochrysis galbana Parke 8701
投稿时间:2014-04-16  修订日期:2014-04-16
DOI:
中文关键词: 球等鞭金藻8701 耐低温机理 超氧化歧化酶 饵料 过氧化物酶 电解质外渗率 海水养殖动物
英文关键词: Isochrysis galbana 8701  low temperature tolerance mechanism  superoxide dismutase  peroxidase
基金项目:国家自然科学基金资助项目(39870146) ; 山东省自然科学基金资助项目( Y95D0144)
作者单位地址
宫相忠 青岛海洋大学海洋生命学院!山东青岛266003 青岛海洋大学海洋生命学院!山东青岛266003
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中文摘要:
      对耐低温品系球等鞭金藻8701在低温下与活性氧清除和伤害有关的几个生理生化指标进行了研究,探讨了其耐低温机理,并与该藻种的低温敏感品系球等鞭金藻3011进行了比较,结果表明,(1)球等鞭金藻8701在低温下(8℃)培养一周,其超氧化物歧化酶(SOD)活性略有下降,但仍保持极高的酶活性,因此能够及时清除低温胁迫产生的过量活性氧,使其细胞内保持较低的膜脂过氧化水平和较低的电解质外渗率,(2)球等鞭金藻3011在低温下胁迫一周,其细胞内的膜脂过氧化水平和电解质在率大大高于球等鞭金藻8701,这与其SOD活性较低,并且在低温下其活性大幅度下降有关,(3)与DOD相比,两种不同品系球等鞭金藻的过氧化物酶(POD)对减轻其低温伤害,提高其耐低能力,不起主要作用。
英文摘要:
      This paper reports several physiological and biochemical changes related to active oxygen injure and scavenging effect in a low temperature resistant strain Isochrysis galbana Parke 8701 under low temperature stress and investigates its low temperature tolerant mechanism in comparison with a low temperature sensitive strain I. galbana Parke 3011. The experimental results showed that : ⑴the activity of superoxide dismutase (SOD) in I. galbana Parke 8701 decreased slightly when cultured at low temperature ( 8 ℃) for a week , but still kept extremely high enzymatic activity , so it could scavenge the extra active oxygen rapidly under low temperature stress and keep a membrane lipid peroxidation in relatively lower level as well as a relatively low electrolytic leakage; ⑵the membrane lipid peroxidation level and electrolytic leakage in I. galbana Parke 3011 were significantly higher than in I. galbana Parke 8701 when stressed under low temperature for a week. This was due to the relatively low SOD activity in the former and its activity rapidly declined; ⑶in comparison with SOD , peroxidase (POD) did not play an important role in relieving the low temperature injury and raising the capacity of low temperature tolerance in these two strains of I. galbana Parke.
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