文章摘要
朱建中.对虾白斑综合征病毒在螯虾动物模型的感染特性[J].水产学报,2001,25(1):
对虾白斑综合征病毒在螯虾动物模型的感染特性
Characterization of shrimp white spot syndrome virus(WSSV) infection in Cambarus proclarkii
投稿时间:2014-04-16  修订日期:2014-04-16
DOI:
中文关键词: 克氏原螯虾 白斑综合征病毒 感染温度 感染途径 继发感染 免疫保护 保存期
英文关键词: Cambarus proclarkii  white spot syndrome virus(WSSV)  infection temperature  infection route
基金项目:
作者单位地址
朱建中 南京农业大学农业部动物疫病诊断与免疫重点开放实验室!江苏南京210095 南京农业大学农业部动物疫病诊断与免疫重点开放实验室!江苏南京210095
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中文摘要:
      应用克氏原螯虾作为动物模型研究对虾白斑综合征病毒(WSSV)的感染增殖特性,涉及感染温度,感染途径,继发感染,半数致死量(LD50),免疫保护及保存期等,结果显示,22-25℃时,接种WSSV的螯虾一般于2-7d内死亡,温度升高对病毒增殖影响不显著,30-32℃时,平均死亡时间2-6d,温度降低对病毒增殖影响较显著,15-19℃时,接种螯虾平均2-10d内死亡,8-10℃时,平均死亡时间3-6d,感染途径分别用腹节肌肉,腹节皮下洲射及口服,均能使螯虾感染发病,而浸泡方式不能使螯虾发病,细菌分离和细菌定量结果表明,寄考于螯虾心脏,肝胰腺内的阴沟肠杆菌在感染后期大量增殖,菌量分别是正常螯虾的25和30倍,形成继发感染,用螯虾心脏,肝胰腺内的阴肠杆菌在感染后期大量增殖,菌量分别是政党螯虾的25和30倍,形成继发梁。用螯虾测定WSSV的LD50为10^-6.5.mL^-1种毒液,将病毒56℃,30min灭活后免疫螯虾,不能使螯虾形成免疫保护,WSSV匀浆液-30℃冻存1年后失活,而-30℃冻存于螯虾体内WSSV保存1年后仍有活力。
英文摘要:
      Crawf ishes ( Cambarus proclarkii ) as animal were infected with white spot syndrome virus (WSSV) for studying various infection characters, including temperature, transfer route, successive infection, median lethal dose ( LD50) , immunity against virus and virus conservation etc. The results showed crawf ishes usually died in 2 - 7d after infection at the temperature of 22- 25 .. , in 2- 10d at 15- 19 .. , in 3- 13d at 8- 10 .. and 2- 6d at 30- 32 .. . Below 5 .. , the infected crawf ishes could remain active for more than one month. By injecting and oral feeding, WSSV could infected crawfishes successfully and there was no significant difference between muscle injection and hypoderm injection. Soaking with WSSV failed to infect crawfishes. Bacteria examination in healthy and diseased crawfishes showed Enterobacter cloacae in the heart and hepatopancreas multiplied 25 and 30 times respectively , indicating that bacteria successive infect ion existed during the virus infection. The LD50 was 10 - 6. 5..mL - 1 WSSV. Crawfishes which were immunized with WSSV inactivated at 56 .. for 30min could not obtain immunity protection from. WSSV f rom homogenate stored at - 30 .. for 1 year could not be recovered but f rom crawfishes stored at - 30 .. for 1 year could be recovered successfully.
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