文章摘要
邓敏.鳜传染性脾肾坏死病毒p31基因结构及序列分析[J].水产学报,2001,25(6):
鳜传染性脾肾坏死病毒p31基因结构及序列分析
Characterization of the p31 gene of infectious spleen and kidney necrosis virus of Siniperca chuatsi
投稿时间:2014-04-14  修订日期:2014-04-14
DOI:
中文关键词: 虹彩病毒 传染性脾肾坏死病毒 p31基因 序列分析 鳜鱼
英文关键词: iridovirus  infect ious spleen and kidney necrosis virus ( ISKNV)  p31 gene  sequence analysis  phylogeny
基金项目:广东省自然科学基金
作者单位地址
邓敏 中山大学生命科学学院 中山大学生命科学学院
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中文摘要:
      报道了鳜传染性脾肾坏死病毒(ISKNV)的p31基因结构及其序列分析.对ISKNVDNAHindmE酶切片段的序列分析结果发现该序列中含有完整的p31基因.ISKNVp31基因完整读码框为675bp,GC含量为49.78%,等电点为7.61,编码一个长为225aa、分子量为25.3 kD的推定蛋白.结构分析发现该基因具有启动子元件TATA box和CAAT motif,下游有反向重复序列可形成茎环,另外还有一段直接重复序列和二联体结构.ISKNV与其它3种虹彩病毒(包括FV3、LCDV-1和EHNV)的p31基因氨基酸序列具有一定的同源性,但ISKNV与它们的同源性不高,序列比较和系统树分析发现ISKNV与蛙病毒属和淋巴囊肿病毒属的病毒都不尽相同.
英文摘要:
      P31 gene of infect ious spleen and kidney necrosis virus ( ISKNV ) of Siniperca chuatsi had been sequenced. The open reading frame of the gene is 675 nucleotides long, encoding a putative protein of 225 amino acids with 25. 3kD. The 5. noncoding region has a TATA box and CAAT motif. A stem loop, a direct repeat sequence and a short dyad are contained downstream of the translation stop codon. The results of comparison of the homology with other iridoviruses p31 genes showed that ISKNV p31 gene has lower identities with others. Alignment of amino acid sequences and a phylogenetic tree of p31 genes of four iridoviruses have been constructed. The results indicated that ISKNV is different from the viruses of the genus Ranavirus and Lymphocystivirus.
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