文章摘要
邹记兴.点带石斑鱼的核型、C带、Ag-NORs[J].水产学报,2005,29(1):
点带石斑鱼的核型、C带、Ag-NORs
The karyotypes,C-bands patterns and Ag-NORs of Epinephelus malabaricus
投稿时间:2014-03-13  修订日期:2014-03-13
DOI:
中文关键词: 点带石斑鱼 核型 C带 Ag-NORs
英文关键词: Epinephelus malabaricus  karyotype  C2bands  Ag2NORs
基金项目:浙江省高等学校青年教师资助计划,宁波大学校科研和教改项目
作者单位地址
邹记兴 宁波大学生命科学与生物工程学院 宁波大学生命科学与生物工程学院
摘要点击次数: 1632
全文下载次数: 1877
中文摘要:
      采用PHA活体注射结合秋水仙素培养,取点带石斑鱼全肾,低渗处理,空气干燥制片法制作染色体标本,对染色体进行Giemsa染色、C带及Ag-NORs等系列研究.结果表明:(1)点带石斑鱼2n=48,核型组成为48t,NF=48,没有异型性染色体分化;(2)在最小一对染色体的着丝粒与其染色体臂之间,靠近着丝粒部位有明显的次缢痕;(3)在间期核中,通过银染表现出核仁的数目为1~4个,1个核仁的间期核数目最高,多达55%,4个核仁的间期核数目占2%;(4)50%有丝分裂中期相能观察到Ag-NORs,Ag-NORs主要出现在第24对同源染色体上,第5对同源染色体也可观察到,但其它染色体上则没有;(5)Ag-NORs的数目在不同的细胞中表现出多态性,数目为1~4个,出现4个Ag-NORs的频率最低(6.8%),出现2个Ag-NORs的频率最高(58.1%);(6)第24对同源染色体近着丝粒的臂内具次缢痕,是Ag-NORs所在的区域,该区域分布有大量的结构异染色质,即Ag-NORs与C带强阳性呈现严格的同步对应;(7)点带石斑鱼所有染色体着丝粒为阳性C带,而且第24对染色体几乎整个染色体臂都呈C带阳性,着色强度与该对染色体上的着丝粒C带相同.最后讨论了核型演化规律和Ag-NORs、C带的发生机制.
英文摘要:
      The chromosome specimens of Epinephelus malabaricus (Bloch &Schneider) were obtained from metaphase of kidney cell by viv2injection of PHA and cultivation of colchicines , hypatoic2air drying technique , and then by studying their Giemsa stain , C2bands and Ag2NORs. The results were as follows : 1) E. malabaricus had a diploid chromosome number of 48 and its karyotype formula was 48t , NF = 48 ,sex chromosome was not found;2) There was a pair of chromosomes with secondary constriction close to the centromere of chromosomet24; 3)1-4nucleoliappearedinthenucleusofinterphase,55%nucleihadlnucleolusandonly2%for4nucleoli;4) Ag2NORs appeared in the chromosome t 24 of 50 %metaphase ,sometimes in the chromosome t5 ,but not in other chromosomes ;5) The Ag2NORs polymorphisms were individually specific ,1 -4 pairs of the number ,and the frequency of 4 Ag2NORs were lowest ; 6) The secondary constrictions and positive C2bands were coincident , close centromere the chromosome , and mass constrictive heterochromatinsappearedinthatregion;7)Allthecentromeresofchromoso(tothe) mesweredarkl(of) y stained C2bands ,and the whole arm of chromosome t 24 and its centromere were same positive C2bands. 8) The evolutive regulation of the karyotype and the developing mechanism of Ag2NORs and C2bands were discussed.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看