文章摘要
宋晓玲.双歧杆菌细胞壁肽聚糖的分离及其对二种海产动物免疫活性的影响[J].水产学报,2005,29(3):
双歧杆菌细胞壁肽聚糖的分离及其对二种海产动物免疫活性的影响
Peptidoglycan isolation from Bifidobacterium thermophilum and its effect on immuno-enzymetic activity of Penaeus japonicus and Paralichthys olivaceus
投稿时间:2014-03-13  修订日期:2014-03-13
DOI:
中文关键词: 日本对虾 牙鲆 双歧杆菌 肽聚糖 免疫酶活性
英文关键词: Penaeus japonicus  Paralichthys olivaceus  Bifidobacterium thermophilum  peptidoglycan (PG)  immuno2enzymatic activity
基金项目:国家高技术研究发展计划(863计划)
作者单位地址
宋晓玲 中国水产科学研究院黄海水产研究所 中国水产科学研究院黄海水产研究所
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中文摘要:
      采用三氯乙酸裂解法分离双歧杆菌细胞壁肽聚糖,得到的肽聚糖约占双歧杆菌细胞干重的8.4%,其中总糖含量约为24.2%,脂肪酸含量约为3.2%,蛋白质含量约为2.4%;氨基酸含量及组分分析结果显示:与N-乙酰胞壁酸羧基连接的肽链上的甘氨酸、谷氨酸、赖氨酸和丙氨酸间的摩尔比为1:0.7:1.1:6.7.将所得肽聚糖经超声波破碎后,制成0.1%和1%两种浓度的悬液,并将相应的缓冲液设置为对照组,分别注射给日本对虾和牙鲆,测定其对日本对虾和牙鲆血清酚氧化酶(PO)、超氧化物歧化酶(SOD)、碱性磷酸酶(ALP)、酸性磷酸酶(ACP)和溶菌酶(LSZ)5种免疫酶活性的影响.结果显示:肽聚糖可显著提高日本对虾血清内的PO活性和LSZ活性,并在96h内一直维持在较高水平;肽聚糖可适当提高日本对虾血清SOD活性,但到72 h又降回原来的水平;肽聚糖可影响日本对虾血清的ALP活性和ACP活性,且两种作用趋势相近,96h内呈逐渐平缓上升的趋势.肽聚糖可显著提高牙鲆血清的LSZ活性,但这种升高具有明显的时效性,72 h后降至原来的水平,且不同浓度的作用效果也有差异.由此可见,肽聚糖可作为免疫增强剂用于提高养殖日本对虾和牙鲆的非特异性免疫水平.
英文摘要:
      Peptidoglycan (PG) was isolated from Bifidobacterium thermophilum by TCA extracting , and its weight was 8. 4 % of dry weight of bacterium. The peptidoglycan contained 24. 2 % glycan , 3. 2 % fatty acid and 2. 4 % protein. The concentrations of Gly , Glu, Lys and Ala, the special amino acid of PG, were 157.2, 102.0, 173.8 and 1 061.1μmol?g -1 , respectively. After chemical compositionofPGwasanalyzed,thebiologicalactivitiesofPGwereexaminedtoo. EffectsofPG,whichhadbeendilutedto 0.1% and 1 % by special buffer and broken up by ultrasonic waves , on the activity of Phenoloxidase (PO) , Superoxide Dismutase (SOD) , Alkaline phosphatase (ALP) , Acid phosphatease (ACP) and Lysozyme (LSZ) of the serum of penaeid shrimp , Penaeus japonicus were studied. The results showed that the activity of PO and LSZ had been markedly enhanced and it could maintain in 96 h , the activity of SOD had been enhanced properly and it would decline back in 72 h , after ip PG. The effects of PG on the activity of ACP and ALP were similar , the enzyme activity was mildly increased in 96h. Except Penaeus japonicus , Paralichthys olivaceus were used as experiment animal too. PG could enhance the activity of SOD of fish serum significantly , but this activity was obviously different as time extending and it fell to the original value after 72 h passed. And different concentrations of PG had different effects. The results indicate that PG can be used as immunostimulant in order to enhance the non2specific immune activity of aquatic shrimps and fishes.
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