文章摘要
黄剑南.赤点石斑鱼神经坏死病毒外壳蛋白全基因克隆与序列分析[J].水产学报,2005,29(3):
赤点石斑鱼神经坏死病毒外壳蛋白全基因克隆与序列分析
Molecular cloning and sequence analysis of complete coat protein gene of nervous necrosis virus from Epinephelus akaara
投稿时间:2014-03-14  修订日期:2014-03-14
DOI:
中文关键词: 赤点石斑鱼 神经坏死病毒 外壳蛋白基因 克隆 序列分析
英文关键词: Epinephelus akaar a  nervo us necrosis virus( NNV)  complete coat protein gene  molecular cloning  sequence analysis
基金项目:国家高技术研究发展计划(863计划),广东省自然科学基金团队项目
作者单位地址
黄剑南 中山大学生命科学学院生物防治国家重点实验室 中山大学生命科学学院生物防治国家重点实验室
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中文摘要:
      
英文摘要:
      Viral nervous necrosis ( VNN) is a worldwide disease among teleost fish. In the mainland of China, VNN was first identified in 2 species of hatchery-reared groupers, Epinep helus akaara and E . coioides . In the present study , samples were collected from larvae of E . akaar a w ith signs of VNN in Dayawan bay which is located in so uthern China. The complete viral coat protein gene was amplified using extracted total RNA as template. The reverse transcription po lymerase chain reaction ( RT-PCR) amplification was performed using primers containing a heterolo gous restriction site for NotI. PCR products were cloned into pcDNA3 vector and sequenced. The results indicated that the coat protein gene of Dayawan iso late ( RG-CN) was 1056 bases in length and contained a single open reading frame ( ORF) of 1017 bases encoding a protein of 338 amino acids. The sequence similarities between the coat protein gene of RG-CN and other 8 isolates of NNV from Dayawan, Taiwan, Japan, Singapore and France were 79. 1%- 99. 5% at the nucleotide level and 83. 7%- 100% at the amino acid level, respectively. The homo logy between RG-CN and the other 5 isolates from groupers was high and relatively lower between RG-CN and guppy nervous necro sis virus ( GNNV) , sea bass nervous necrosis v ir us ( DIEV) , but more divergences existed between RG-CN and striped jack nevo us necro sis virus ( SJNNV) . Compared w ith SJNNV, RG-CN and the other 7 iso lates all lacked 6 nucleotides and 2 amino acids in the same po sitions. Based on the result of molecular phylogenetic analysis, Dayawan iso late belongs to red- spotted grouper nervous necrosis virus ( RGNNV) genotype.
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