文章摘要
林蠡.赤点石斑鱼病毒性神经坏死症的组织病理和电镜观察[J].水产学报,2005,29(4):
赤点石斑鱼病毒性神经坏死症的组织病理和电镜观察
Histopathological examination and electron microscopy observation of viral nervous necrosis in red-spotted grouper, Epinephelus akaara
投稿时间:2014-03-11  修订日期:2014-03-11
DOI:
中文关键词: 赤点石斑鱼 Beta诺达病毒 病毒性神经坏死症
英文关键词: Epinepheelus akaara Betanodarins viral nervous necrosis(VNN)
基金项目:国家高技术研究发展计划(863计划),国家留学基金委留学基金
作者单位地址
林蠡 中山大学生命科学学院生物防治国家重点实验室 中山大学生命科学学院生物防治国家重点实验室
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中文摘要:
      用逆转录-聚合酶链式反应(RT-PCR)检测患病赤点石斑鱼苗,呈Beta诺达病毒阳性.光学显微镜下观察到病鱼的脑、视网膜、脊髓有空泡.在脑部,空泡主要分布在端脑、间脑和小脑.受感染的细胞明显收缩、致密变化和嗜碱性.包涵体常为圆形,大小不一.透射电镜下,在感染细胞的细胞质可观察到含有病毒粒子的致密体.病毒粒子呈等面体,无外膜,直径为25~28 nm,随机分布在细胞质或在致密体内排列成晶格状.致密体大小不一.偶尔观察到较大致密体的外膜已破裂,病毒粒子被释放到细胞质.
英文摘要:
      A specific band of Betanodavirus RNA2 was detected from red-spotted grouper( Epinephelus akaara) larvae by reversetranscription polymerase chain reaction ( RT-PCR). Under light microscopy, the brain, retina, and spinal cord of affected fish showed conspicuous vacuolation. In the brains, vacuoles were mainly apparent in the telencephalon, the diencephalon and the cerebellum. Affected cells were pyknosis accompanying marked shrinkage and basophilia. Inclusion bodies were usually round and different in size. Transmission electron microscopy revealed affected cells containing dense bodies filled with numerous viral particles in the cytoplasm. Virions were 25 - 28 nm in diameter, icosahedral and non-enveloped. Virions were distributed randomly in the cytoplasm or arranged in crystalline arrays in the dense bodies. The sizes of dense bodies were different. Occasionally, the unit membrane surrounding the larger bodies had ruptured, allowing the viral particles to escape into the cytoplasm.
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