文章摘要
吕道远.金鱼DEAD-box家族基因p68和p110在配子发生中的表达特征[J].水产学报,2005,29(5):
金鱼DEAD-box家族基因p68和p110在配子发生中的表达特征
Expression pattern of Carassius auratus DEAD-box family genes p68 and p110 during gametogenesis
投稿时间:2014-03-09  修订日期:2014-03-09
DOI:
中文关键词: 金鱼 原位杂交 DEAD-box p68基因 p110基因 卵子发生 精子发生
英文关键词: Carassius auratus in situ hybridization DEAD-box p68 gene p110 gene oogenesis spermatogenesis
基金项目:国家高技术研究发展计划(863计划),国家自然科学基金
作者单位地址
吕道远 武汉大学生命科学院发育生物学教育部重点实验室 武汉大学生命科学院发育生物学教育部重点实验室
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中文摘要:
      利用组织原位杂交技术,以地高辛标记的反义RNA为探针,检测了金鱼(Carassius auratus) DEAD-box家族基因p68和p110在卵子发生及精子发生中的表达分布特点.结果表明:在金鱼配子发生中,p68 基因在各个时期的卵母细胞均有表达,Ⅰ、Ⅱ期卵母细胞中的表达水平较Ⅲ、Ⅳ期卵母细胞中更高.p68 基因的表达只限于精原细胞和初级精母细胞中.p110基因在金鱼精子发生各阶段持续表达,其中精原细胞和初级精母细胞中表达水平较高;在卵子发生中,Ⅰ期卵母细胞中没有p110 RNA阳性信号,Ⅱ期卵母细胞中信号较为强烈,但在Ⅲ、Ⅳ期卵母细胞中杂交信号明显减弱.
英文摘要:
      In order to study the potential functions of DEAD-box family genes p68 and p110 in germ cell proliferation and diffemtiation, using in situ hybridization on tissue sections with DIG labeled antisense RNA probe, we detected the location of goldfish (Carassius auratus) DEAD-box family genes p68N and p110 RNA during gametogenesis. The results are as follows: during goldfish oogenesis, the expression of p68 RNA was observed in oocytes at all stages, and its expression was higher in stage Ⅰ , Ⅱ oocytes than in stage Ⅲ, Ⅳ oocytes. The expression of p110 RNA could not be found in stage Ⅰ oocytes and strong signals could be detected in stage Ⅱ oocytes, but the signals became much weaker in stage Ⅲ, Ⅳ oocytes. During goldfish spermatogenesis, the expression of p68 RNA could only be detected in spermatogonia and primary spermatocytes. The expression of p110 RNA was visible throughout spermatogenesis, and its expression was much higher in spermatogonia and primary spermatocytes than in spermatids. Based on the active expression of p68 and p110 during goldfish gametogenesis, we speculated that they may play potentially important roles in germ cell development of both sexes.
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