文章摘要
孙博.海湾扇贝个体间单向人工授精的分子生物学验证[J].水产学报,2006,30(5):713~719
海湾扇贝个体间单向人工授精的分子生物学验证
Molecular verification of fertilization between bay scallop individuals
投稿时间:2008-05-14  修订日期:2008-05-14
DOI:10.3724/SP.J.00001
中文关键词: 海湾扇贝  人工授精  杂交  家系  RAPD  分子标记
英文关键词: Argopecten irradians irradians  fertilization  hybridization  family  RAPD  molecular marker
基金项目:山东省科技发展计划项目(022110107)
作者单位E-mail
孙博 中国科学院海洋研究所 liuxiao@ms.qdio.ac.cn 
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英文摘要:
      Hybrid family of Argopecten irradians irradians was created by fertilization between two northern bay scallop individuals. Two families were analyzed in this study. The first family, Pa-Pb, is a pair mating between two scallops named Pa and Pb, while the second one crossed by individuals of Y1 and P0. Marker inheritance and segregation were studied in the 10 progenies of each family by randomly amplified polymorphic DNA (RAPD) analysis. 102 RAPD primers were first screened by parental animals of both families. Only the primers with polymorphisms between the two parental animals in each family were select ed for further analysis. In both families, parents and 10 progeny were nalyzed with selected primers. In family Pa-Pb, a total of 122 bands generated from 12 selected primers. 37 of them were polymorphic between two parents. The maternal Pa of this family had 17 molecular markers while paternal Pb had 20 markers. In Y1-P0,95 bands were produced by 10 selected primers. 32 bands were polymorphic between maternal Y1 and paternal P0, who had 17 and 15 molecular markers respectively. In both families, each progeny analyzed in this study had at least 8 maternal markers and 5 paternal markers. Based on segregation patterns at all markers analyzed, we concluded that none of the progeny analyzed were from self-fertilization, and one way hybridization between two individuals was successful in both of the two bay scallop families.
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