文章摘要
柳广东.日本绒螯蟹ND2基因全序列测定与分析[J].水产学报,2005,29(5):
日本绒螯蟹ND2基因全序列测定与分析
The complete sequence of the mitochondrial ND2 gene of Eriocheir japonica
投稿时间:2014-03-10  修订日期:2014-03-10
DOI:
中文关键词: 日本绒螯蟹 ND2基因 全序列
英文关键词: Eriocheir japonica ND2 gene complete sequence
基金项目:教育部重点实验室基金
作者单位地址
柳广东 中国海洋大学海水养殖教育部重点实验室 中国海洋大学海水养殖教育部重点实验室
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中文摘要:
      动物线粒体DNA(mtDNA)具有比核DNA的进化速率快等特点,而被广泛地应用于生物的起源、演化和种群遗传学研究[1].完整的线粒体基因组包括37个基因:13个编码疏水性蛋白质亚基基因、22个tRNA基因、2个rRNA基因.各基因排列紧密,不含内含子,且有些基因区域出现重叠.Zardoya和Meyer考察了脊椎动物mtDNA上13个蛋白编码基因,认为ND4、ND5、ND2、Cytb和COI基因包含良好的系统发育信息[2].从Anderson等测定了人的线粒体基因组全序列开始[3],目前已有多种动物的线粒体DNA全序列或部分基因序列被测定[4-12].
英文摘要:
      Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2 (ND2) gene in mitochondrial tRNAMet and tRNAvrp gene respectively, and the complete sequence of the ND2 gene of Eriocheir japonica was determined. The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp. The A, T, G, C contents of the gene were 28.54% (288 bp), 44.20% (446 bp), 8.23% (83 bp), and 19.03% ( 192 bp) respectively; and the sequence was AT rich and biased. The start and stop codons were ATC and A respectively. The homology analysis of nucleotide sequence showed that the similarities in NEE gene between Eriocheir japonica and other crustacean were comparatively low, which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species.
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