文章摘要
颜素芬.中国龙虾早期叶状幼体肝胰腺的显微与超微结构[J].水产学报,2005,29(6):
中国龙虾早期叶状幼体肝胰腺的显微与超微结构
Micro-ultrastructure of hepatopancreas of phyllosoma larvae in Panulirus stimpsoni
投稿时间:2014-03-09  修订日期:2014-03-09
DOI:
中文关键词: 中国龙虾 叶状幼体 肝胰腺 组织学 组织化学 超微结构
英文关键词: Panulirus stimpsoni phyllosoma larvae hepatopancreas histology histochemistry ultrastmcture
基金项目:福建省自然科学基金
作者单位地址
颜素芬 集美大学水产生物技术研究所 集美大学水产生物技术研究所
摘要点击次数: 1465
全文下载次数: 0
中文摘要:
      利用光镜和电镜研究中国龙虾发育早期(Ⅰ~Ⅳ期)叶状幼体的肝胰腺的形态学、组织学、组织化学和超微结构.结果表明:肝胰腺左右对称各分成3叶,每叶由许多分支的肝胰腺管组成.根据其结构与功能的不同,腺管上皮细胞可分为泡状细胞(B细胞)、纤维细胞(F细胞)、吸收细胞(R细胞)和胚性细胞(E细胞)四种类型.各类细胞的游离面具微绒毛.B细胞有明显胞饮现象,内含一个大消化泡和许多液泡,起吸收和细胞内消化作用.R细胞内线粒体、内质网较丰富,含少量脂滴,具吸收与储存养分的功能.F细胞具有发达的粗面内质网、丰富的游离核糖体和酶原颗粒,行细胞外消化作用.E细胞胞器不发达,是细胞分化的起点.组织化学研究表明:各类细胞大多有多糖类物质,R细胞含少量糖原和脂类,F细胞内含丰富蛋白质,其它细胞含量次之.本文还讨论了肝胰腺细胞的类型及各细胞结构与功能的关系.
英文摘要:
      The phyllosoma larvae( Ⅰ - Ⅳ ) of Patudirus stimpsoni of early development were collected from marine farm of Fisheries College of Jimei University from July to Sep. from 2000 to 2001. The histology, histochemistry and ultrastructure of their hepatopancreas were studied with light and electron microscopes. The results show that the hepatopancreas are made of two parets (right and left) and each part can be divided into three lobes that consist of many hepatopancreatic tubules, which increases in length and amount with the development of larva. Hepatopancreas connects the pyloric stomach and the midgut with two common gland duct (right and left). The epithelium cells of hepatopancreatic tubules are classified into blister-like cells (B-cells), resorptive cells (R- cells), fibrillar cells(F-cells) and embryonic cells(E-cells) based on structure and function. The surface of all type cells has microvilli and these cells are located on a thin basement membrane. B-cells are mainly located in the proximal regions of the tubules, their volumes are the biggest and shapes are not regular, nuclei are crescent. B-cells have a large digestive vacuole, the denes cytoplasm, pinocytofic vesicles, small mitochondda exist in an apical complex body between the microvilli and large digestive vacuole. There are rough endoplasmic reticulum(RER) in basement of the cells. R-cells are the most numerous cell type, their nuclei are near round and located in the basement of cells. In some R-cells( called Rl-cell),no vacuoles are observed while some R-cells( called R2-cell)have many vacuoles(under light microscope). Mitochondria with moderate electron density matrix are developed and have many ridges, and RER and free ribosome are rich in Rl-cells where plnocytotic vesicles and dense granules were rarely observed. But in R2-cells, electron density of mitochondria is low and ridges are a few, there is the extensive and well-developed smooth endoplasmic reticulum (SER) containing material of high density in the basal region of the cell, some vesicles appeared around Golgi bodies and lipid droplets are a few or are forming in R2-cells. F-cells are mainly located in the middle regions of the tubules and scattering among B-cells and R-cells. It is strongly basophile, their nuclei are large and located in the middle regions of cells. RER, mitochondria and ribosome are very rich and zymogen granules aggregated in the apical cytoplasm. E-cells are located in the blind end of the tubules and undifferentiated, their volumes are small and a round or oval nucleus exists in middle regions of cells, and show a high ratio of nucleus to cytoplasm, cytoplasmic organelles are undeveloped in the E-cells. The study of histochemistry showed that four types of hepatopancreatic cells contained generality polysaccharides; there are some glycogen and lipid droplets in R-cells; the proteinic content is rich in the F-cells but less in other type of cells. The relation between the structure and function of four types of hepatopancreatic cells of the phyllosoma larvae was studied too in this paper. It is generally believed that the main absorptive and digestive function of the gland is carried out by the B-cells which ingest nutrients by pinocytosis and process them in a large digestive vacuole;the R-cells absorb soluble material from the lumen by diffusion and store them as lipid and glycogen in the cytoplasm;F-cells synthesize zymogen granules which are exocytosed into the tubule lumen and carry out function of extracellular digestion. E-cells divide by mitosis to differentiate into other tvoe of cells.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看