文章摘要
陆宏达.中华绒螯蟹血细胞数及离体后形态学变化[J].水产学报,2006,30(4):454~462
中华绒螯蟹血细胞数及离体后形态学变化
Counting and morphological changes in vitro of haemocytes in Eriocheir sinensis
投稿时间:2008-05-28  修订日期:2008-05-28
DOI:10.3724/SP.J.1231
中文关键词: 中华绒螯蟹  血细胞  计数  形态学变化  血凝
英文关键词: Eriocheir sinensis  haemocyte  counting  morphological changes  coagulation
基金项目:上海市自然科学项目(02NK05)
作者单位E-mail
陆宏达 上海水产大学生命科学与技术学院 hdlu@shfu.edu.cn 
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中文摘要:
      中华绒螯蟹循环血液中血细胞总数为(13357±7196) cells·mm-3,其中大颗粒细胞所占(49.8±7.5)%,其长轴(16.6±1.1) μm,短轴(10.2±0.6) μm;大小颗粒中间型细胞所占比例(20.6±5.3)%,其长轴(13.1±1.2) μm,短轴(8.4±0.5) μm;小颗粒细 胞所占比例(29.3±4.8)%,其长轴(12.4±1.2) μm,短轴(8.1±0.4) μm;无颗粒细胞所占比例(0.2±0.2)%,其长轴(11.3±1.3) μm,短轴(8.9±1.1) μm。血液离体后,在血液完全凝固的(97.7±25.3) s时间内,只有无颗粒细胞和小颗粒细胞形态结构发生变化,细胞及其胞核立即膨大,核质比迅速增大,细胞膜破损崩解,胞浆溢出,血液完全凝固后这些细胞形态结构继续变化,主要表现为胞质内线粒体、内质网等细胞器扩张溶解消失,小颗粒细胞中的小颗粒内含物渗出或溢出,颗粒空泡化或崩解,细胞核固缩等变化过程 。而大小颗粒中间型细胞和大颗粒细胞形态结构的变化明显慢于前两种血细胞,在细胞离体后5 min时,细胞和细胞核才开始出现膨大,并逐渐出现细胞膜、线粒体、内质网溶解消失,颗粒空泡化或崩解以及细胞核固缩等形态结构的变化。从血液离体后血细胞形态结构随时间变化的特点,推测无颗粒细胞和小颗粒细胞可能类似于脊椎动物的血小板,通过释放胞内凝血物质参与血凝的级联反应。
英文摘要:
      The number of haemocytes per cubic milliliter haemolymph in Eriocheir sinensis was (13357±7196). The percentage of large granular cell (LGC) was 49.8% and its size was (16.6±1.1) μm×(10.2±0.6) μm; The percentage of large and small granular intermediate cell (LSGC) was 20.6% and its size was (13.1±1.2) μm×(8.4±0.5) μm; The percentage of small granular cell (SGC) was 29.3% and its size was (12.4±1.2) μm×(8.1±0.4) μm; The percentage of agranular cell (AGC) was 0.2% and its size was (11.3±1.3) μm×(8.9±1.1) μm. The complete coagulation time of haemolymph in Eriocheir sinensis was (9 7.7±25.3) s, during which only AGC and SGC displayed cytolysis. The main orpho logical and structural changes of AGC and SGC were that they and their nucleisw elled immediately after haemolymph leaving body, the ratio of nucleus to ytopla sm increased rapidly, membranes ruptured and the cytoplasm moved out from the inside of cell. After the complete coagulation of haemolymph, the main changes of AGC and SGC were that their mitochondria and endoplasmic reticulum ruptured, dissolved and disappeared gradually, the substance in the small granules of SGC moved out, the electron density in small granules decreased, the granules vacuolated and disappeared gradually, and nucleus became pyknosis. The cytolysis of LSGC and LGC started 5 min later after haemolymph leaving body and was slower than the cytolysis of AGC and SGC. The main morphological and structural changes of LSGC and LGC were that they and their nuclei swelled, membrane, mitochondria and end oplasmic reticulum ruptured, dissolved and disappeared gradually, large granules in LSGC and LGC vacuolated and disappeared gradually, and nucleus became pyknosis. From the characteristics of AGC and SGC morphological changes after haemolymph leaving body, it was inferred that they released some coagulative factors to get into the coagulative reactions which might be similar to that of vertebrate platelets.
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