文章摘要
卢彤岩.达氟沙星对史氏鲟红细胞抗氧化功能及微核形成的影响[J].水产学报,2006,30(5):591~596
达氟沙星对史氏鲟红细胞抗氧化功能及微核形成的影响
Effect of danofloxacin on antioxidant function and micronucleus formation of erythrocyte in Acipenser schrencki
投稿时间:2008-05-07  修订日期:2008-05-07
DOI:10.3724/SP.J.1231
中文关键词: 史氏鲟  红细胞  抗氧化  微核  达氟沙星
英文关键词: Acipenser schrenscki Brand  erythrocyte  antioxidation  micronucleus  danofloxacin
基金项目:科技部“十五”攻关计划(2001BA505B0506)
作者单位E-mail
卢彤岩 中国水产科学研究院黑龙江水产研究所 lutongyan@hotmail.com 
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中文摘要:
      2004年5月至7月间,用20 mg·kg-1、50 mg·kg-1和100 mg·kg-1剂量对史氏鲟经口灌服达氟沙星20 d,分别于第5、10、15 和20天定红细胞SOD、CAT、Na+、K+ ATP酶、GST、GSH PX等抗氧化酶活性及丙二醛(MDA)含量,以评价达氟沙星对史氏鲟抗氧化防御功能的影响,同时进行红细胞微核率和总核异常率的测定。结果表明实验第5天各实验组红细胞SOD活性均显著高于对照组;各实验组红细胞中CAT的活性明显高于对照组;红细胞Na+、K+ ATP酶活性没有明显的改变。实验第20天时100 mg·kg-1剂量组GSH PX活性明显高于对照组及其他剂量组(P<0.05);GST活性及MDA含量没有明显的影响。达氟沙星在实验剂量内对史氏鲟红细胞核微核率没有明显的影响,但核异常率呈明显升高。
英文摘要:
      The effects of danofloxacin by oral administration for 20 days at dosage of 20,50 and 100 mg·kg-1 on the toxicity of Acipenser schrencki were studied. The activity of superoxide dismutase(SOD) ,catalase(CAT),Na+,K+ ATPase,glutathione S transferase(GSH PX),glutathione peroxidase(GST) and Maleic Dialdehyde(MDA) content were investigated by kit, so as to appraise security of danofloxacin used in Acipenser schrencki. Meanwhile blood smears were prepared to investi gated the micronucleus and nuclear anomalies rate. The results showed:the SOD activity in erythrocyte remains higher level than that of control group;CAT activity in erythrocyte is higher than control group obviously;The activity of Na+, K+ ATPase in erythrocyte is not significant compared with control group;The GSH PX activity in erythrocyte of 100 mg·kg-1 is higher than other test groups(P<0.05);the MDA concentration in erythrocyte remains unchanged;the activity of GST inerythrocyte is not affected by danofloxacin;The erythrocyte micronucleu s remained stable, but nuclear anomalies in test group rose markedly compared with control group. The result showed that the nuclear anomalies are the highest at 10th day, then began to decrease but still is higher obviously than control group. The result showed that danlfloxacin affected SOD,CAT,GSH PX activity, does not changed the Na+, K+ ATPase,GST activity and MDA content. The nuclear anomalies rate of test fish is higher than control group.
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