文章摘要
沈美芳.酶联免疫法测定水产品中呋喃唑酮代谢物AOZ的残留[J].水产学报,2006,30(4):520~524
酶联免疫法测定水产品中呋喃唑酮代谢物AOZ的残留
Enzyme linked immunosorbent assay(ELISA) for the quantitative analysis of furazolidone residues in aquatic products
投稿时间:2008-05-30  修订日期:2008-05-30
DOI:10.3724/SP.J.00001
中文关键词: 水产品  呋喃唑酮代谢物  酶联免疫法
英文关键词: aquatic products  furazolidone metabolotes  enzyme linked immunosorbent assay (ELISA)
基金项目:江苏省自然科学基金(BK2005103)
作者单位E-mail
沈美芳 农业部渔业产品质量监督检验测试中心江苏省水产质量检测中心 mfshen2005@yahoo.com.cn 
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中文摘要:
      运用酶联免疫法对水产品中呋喃唑酮代谢物残留测定的方法进行研究。结果表明,稀释倍数对回收率影响显著,而乙酸乙酯提取次数、光照条件和放置时间对测定结果无显著影响。向样品中分别添加0.60、2.00、6.00 μg·kg-1 3个浓度水平的呋喃唑酮代谢物时,平均回收率分别为99.2%、96.0%和100.0%;批内变异系数为1.76%~12.57%,批间变异系数为5.43%~8.58%,最低检测限为0.3 μg·kg-1。该方法灵敏度高,重复性较好, 适用于水产品中呋喃唑酮代谢物残留的筛选。
英文摘要:
      A method of ELISA for the determination of furazolidone metabolites residues in aquatic products is established in this paper. There were no literatures about determination of AOZ residues in aquatic products by Enzyme linked Immunosorbent assay (ELISA) at home. Samples were derivatived with 2 nitrobenzaldehyde and extracted with ethyl acetate. Then they were purified with n hexane and determinated by ELISA. In the paper, effects of different disposal conditions including dilution factors, extraction times, illumination conditions and shelf time to recovery were researched. By determination on Eriocheir sinensis, Micropterus salmoides and Procambarus clarkii spiked with AOZ, recovery, precision and detection limit of the method were researched by single laboratory validation approach as well as between laboratory comparisons. Results show that there is a remarkable effect of dilution factors on the recovery while no such effect is displayed for extraction times by ethyl acetate, illumination conditions and shelf time. So the best disposal condition is that dilution factor and extraction time are both one. The average recoveries are 99.2%,96.0%and 100.0% respectively when the samples are spiked with 0.60 μg·kg-1,2.00 μg·kg-1 and 6.00 μg·kg-1 of AOZ. The detection limit is 0.3 μg·kg-1. The relative standard deviations(n=3) for intra assay are 1.76%-12.57% and coefficients of variation for inter assay are 5.43%-8.58%. Three positive samples analyzed by ELISA are confirmed by LC MS and no false positive samples are found. The method is sensitive with a fairly good rep roducibility and suitable for the determination of furazolidone metabolites(AOZ) in aquatic products.
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