文章摘要
尹洪滨.俄罗斯鲟的细胞遗传学分析[J].水产学报,2006,30(2):
俄罗斯鲟的细胞遗传学分析
Cytogenetic analysis of Acipenser gueldenstaedti Brandt
投稿时间:2014-03-08  修订日期:2014-03-08
DOI:
中文关键词: 俄罗斯鲟 核型 DNA含量 倍性
英文关键词: Acipenser gueldenstaedti karyotype DNA content ploidy
基金项目:科技部科技基础专项基金项目(主要水产养殖品种种质资源收集、整理、保存)
作者单位地址
尹洪滨 中国水产科学研究院黑龙江水产研究所 中国水产科学研究院黑龙江水产研究所
摘要点击次数: 1262
全文下载次数: 983
中文摘要:
      采用体内注射PHA和秋水仙素,肾细胞短期培养,常规空气干燥法制备俄罗斯鲟(Acipenser gueldenstaedti Brandt)的染色体,对其肾细胞染色体数目统计分析表明,俄罗斯鲟染色体组由236?2条染色体组成,其中具有明显着丝点的染色体为120条,其余为小染色体(点状染色体);核型公式为80m+16sm+24st,t+116mc,NF=332;采用德国PartecpasⅢ型流式细胞分析仪,以鸡红细胞为标准DNA(含量为2.3Pg?N^-1),测定俄罗斯鲟体细胞DNA含量为12.24pg?N^-1,与染色体分析结果比较倍性一致。综合以上两项结果可得出,俄罗斯鲟为八倍体鱼。
英文摘要:
      Metaphase chromosome specimens of Acipenser gueldenstaedti Brandt injected with PHA and colchicin, were prepared from short-term culture of kidney cells with air-drying technique. It diploid chromosome number is 2n = 236?2. The kinetic bodies of the 120 chromosomes were watched and the others are microchromosome. Karyotype consists of 80m + 16sin + 24st, t + 116mc,NF = 332. Diploid nucleus DNA content was measured from the somatic cell of Acipenser gueldenstaedti Brandt, using flow cytometer ( Partec pasⅢ ,made in Germany) and the DNA content (2.3 pg?N^-1) of erythrocytes of chick ( Gallus sp) as standard. It DNA content is 12.24 pg?N^-1, which corresponds with the result measured chromosome number as ploidy. Synthesizing above results, the conclusion was obtained that Acipenser gueldenstaedti Brandt is octalpoid. Compared with the reports about sturgeons and paddlefishes, the results of this paper were identical with the one measured by Song Shu-xiang et al. about A. schrenckii(DNA content is 11.73 pg ? N^-1 ; chromosome number is 238 ?) using flow cytometer, but is different from the ones of P. spathuta (3.96 pg), P. gladius (4. 11 pg), A. dabryanus (8. 26 pg), A. schrenckii (6. 07 pg), A. sinensis (9. 07 pg) by Zhang siming using microspectrophotometer. These differences are relevant to the methods used ( flow cytometer or microspectrophotometer), standard DNA(erythrocytes of chick or human lymphocyte)and the DNA absolute content (for example, the DNA absolute content of erythrocytes of chick is 2.3 pg or 3.22 pg).
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看