文章摘要
王桂芹.饲料蛋白对翘嘴红鲌蛋白质周转代谢的影响[J].水产学报,2006,30(2):
饲料蛋白对翘嘴红鲌蛋白质周转代谢的影响
The.effect of dietary protein on protein turnover of Erythroculter ilishaeformis juveniles
投稿时间:2014-03-08  修订日期:2014-03-08
DOI:
中文关键词: 翘嘴红鲌 蛋白质水平 大豆蛋白 蛋白质周转代谢 肌肉 肝胰脏
英文关键词: Erythroculter ilishaeformis dietary protein level soybean protein protein turnover muscle hepatopancrease
基金项目:浙江省重大科技攻关项目(2002C12016);浙江省湖州市科技重点项目(2003GNN)
作者单位地址
王桂芹 上海水产大学生命科学与技术学院 上海水产大学生命科学与技术学院
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中文摘要:
      选择健康的翘嘴红鲌(体重12.84?0.60g)为试验鱼,以红鱼粉为蛋白源,配制5个蛋白水平(31.04%、35.5l%、40.89%、46.62%、50.33%)的等能、等必需氨基酸(EAA)平衡关联度的半精制饲料;又以豆粕替代鱼粉,大豆蛋白分别替代0、13.5%、27%、40.5%、54%的鱼粉蛋白,配制5个EAA关联度的等蛋白(40%)、等能(20MJ?kg^-1)的半精制饲料,探讨饲料蛋白水平和大豆蛋白对鱼粉蛋白的替代对翘嘴红鲌肌肉和肝胰脏蛋白质周转代谢的影响。8周饲养结果表明,饲料蛋白水平对翘嘴红拍的特定增重率(SGR)具有显著性影响(P〈0.05),40.89%饲料蛋白组的SGR显著高于31.04%、35.51%饲料蛋白组(P〈0.05),但与46.62%和50.33%饲料蛋白组没有显著性差异(P〉0.05);饲料蛋白水平对白肌、肝胰脏蛋白质生长率具有相似的影响,40.89%饲料蛋白组的白肌、肝胰脏蛋白质生长率分别达到2.13%?d^-1,显著高于31.04%和35.51%两组(P〈0.05),但与46.62%和50.33%饲料蛋白组无显著性差异(P〉0.05)。饲料蛋白水平的增加促进了翘嘴红鲌的生长和蛋白质的合成。肌肉蛋白质合成率(Ks)、蛋白质降解率(Kd)随饲料蛋白水平的增加而增加(P〈0.05),肌肉蛋白质的增加归因于蛋白质合成的增加较降解的增加更占优势,蛋白质的沉积率在适宜蛋白水平时最高。当大豆蛋白分别替代13.5%、27.0%、40.5%的鱼粉蛋白时,翘嘴红鲌的SGR与对照组差异不显著(P〉0.05),而且都显著高于54.0%替代组(P〈0.05)。白肌蛋白质生长率(Kg)也显著受到大豆蛋白替代的影响(P〈0.01),当饲料中大豆蛋白对鱼粉蛋白替代量为40.5%时,与对照组相比,Kg极显著下降(P〈0.01)。随着饲料中大豆蛋白对鱼粉蛋白替代量的增加,白肌Ks随之降低,当饲料中大豆蛋白对鱼粉蛋白替代量达54%时,与对照组相比,肌肉Ks显著下降(P〈0.05)。白肌Kd与白肌Ks的变化趋势相似(P〈0.1),而PDE没有受到饲料中大豆蛋白对鱼粉蛋白替代的影响(P〉0.05)。可见随着大豆蛋白对鱼粉蛋白替代量的提高,氨基酸平衡关联度逐渐降低,蛋白质合成逐渐降低(P〈0.05),生长下降,饲料必须氨基酸平衡程度的变化没有改变蛋白质的沉积效率。
英文摘要:
      A series of growth experiments was conducted to examine the effect of dietary protein on protein systhesees parameters [protein synthesis rate (Ks), protein degradation rate (Kd) and protein accumulation rate (Kg) and protein deposition efficiency (PDE) ] in muscle and hepatopancras of topmouth culter, Erythroculter ilishaeformis Bleeker juveniles by using brown fishmeal as the protein source in formulated diets. Tested fish with initial weight ( 12.84 ?0.60 g wet weight)were fed 5 isoenergetic(20 MJ?kg^-1 gross energy ) and the same relativity of essential amino acid balance experimental semi-purified diets containing different protein levels (31.04% ,35.51% ,40.89% ,46.62% ,50.33%dry matter ) in 5% increments. And five isonitrigenous (40% protein) and isoenergefic(20 MJ?kg^-1 gross energy) and different relativity of essential amino acid balance experimental semi-purified diets with soybean protein replacing 0%, 13.5% ,27% ,40.5% ,54% of fish meal protein were formulated. After 8-week trial, The results showed that with increasing dietary protein level, specific growth rate was significantly influenced by dietary protein levels ( P 〈 0.05). Specific growth rate of the fish fed the 40.89% protein diets was significantly higher than that of the fish fed 31.04% and 35.51% protein diets ( P 〈0.05), but them was no significant difference from that of the fish fed the 46.62% and 50.33% protein diets ( P 〉 0.05), Effect of dietary protein levels on protein accumulation rate in muscle followed the similar pattem as that of hepatopancras, protein accumulation rate in muscle and hepatopancras in fish fed the 40.89% protein diets were 2.13%?d^-1 respectively. The specific growth rate of the fish fed the 40.89% protein diets was significantly higher than that of the fish fed 31.04% and 35.51% protein diets ( P 〈 0.05), but them was no significant difference from that of the fish fed the 46.62% ,50.33% protein diets (P〉0.05). Protein synthesis rate (Ks) and protein degradation rate (Kd) in white muscle increased with the ubcrease of dietary protein level( P 〈 0.05) ,protein increase in white muscle was much more due to the increase of Ks than Kd, the muscular protein deposition efficiency ( the proportion of growth to synthesis) had a maximal value in optimistic dietary protein level. When the replacement level for fish meal protein with soybean protein was 13.5%, 27%, 40.5%, them were no significant difference in specific growth rate among the diets compared with control group( P 〉 0.05) ,and were significantly higher than specific growth rate in fish fed with 54% replacement of soybean protein ( P 〈0.05). Replacement of fish meal by soybeaa cake also affected significantly the protein accumulation rate (Kg) in white muscle( P 〈 0.05). When the replacement level for fish meal protein with soybean protein was 40.5%, there was significant decrease of Kg compared with control group( P 〈0.05). When the replacement level for fish meal protein with soybean protein was 54.0%, there was significant decrease of Ks compared with control group( P 〈0.05). In white muscle, increased replacement level for fish meal protein with soybean protein did not change PDE ( P 〉 0.05 ). With the increase of replacement level for fish meal protein with soybean protein, worse EAA balance decreased gradually with the growth, but the change of EAA pattern in diets did not affect PDE of white muscle.
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