文章摘要
鄢庆枇.不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响[J].水产学报,2006,30(2):
不同环境条件对溶藻弧菌粘附大黄鱼肠粘液的影响
Influence of environmental factors on the adhesion of Vibrio alginolyticus to the intestinal mucus of Pseudosciaene crocea
投稿时间:2014-03-08  修订日期:2014-03-08
DOI:
中文关键词: 溶藻弧菌 大黄鱼 粘附作用 粘液
英文关键词: Vibrio alginolyticus Pseudosciaene crocea adhesion mucus
基金项目:国家863计划项目(2002AA639600);福建省自然科学基金(B0410022);福建省青年创新基金(2002J037)
作者单位地址
鄢庆枇 集美大学水产学院 集美大学水产学院
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中文摘要:
      研究了不同环境条件下溶藻弧菌对大黄鱼肠粘液的粘附作用。采用细菌计数法测定溶藻弧菌的粘附作用。粘附的细菌经SYBR GreenⅠ染色后在荧光显微镜下观察,用数码相机拍照后在电脑上计数。试验结果表明,溶藻弧菌对大黄鱼肠粘液的粘附量随菌浓度的升高而升高,并在1—1.5h内趋于饱和;粘附作用在15—30℃、pH偏酸时较强;盐度在5—35范围内对前肠粘液的粘附作用影响不明显,后肠粘液的粘附作用在此范围内随盐度增大而加强,在盐度为0时,溶藻弧菌对前、后肠粘液都无粘附作用;56℃热处理5min及60℃处理1h均能大幅减弱溶藻弧菌对两种肠粘液的粘附作用,表明溶藻弧菌表面的某些热敏结构在粘附作用中起着重要作用。根据以上结果,可以认为溶藻弧菌能够很好地粘附于大黄鱼肠粘液层,其粘附作用受温度、盐度、pH值等环境因子影响很大,溶藻弧菌表面的某些热敏结构可能在粘附过程中起着重要作用。研究结果表明,溶藻弧菌对大黄鱼肠粘液的粘附作用是可控制的,这对于鱼类养殖疾病的控制有一定的参考价值。
英文摘要:
      The mucus layer is the most outer defence barrier of fish. The bacterial attachment to mucus layer is a critical step in the host colonization and subsequent occurrence of infection. Adhesion of Vibrio alginolyticus to the intestinal mucus of Pseudosciaene crocea has been investigated in order to understand the characteristics and mechanisms involved in the adherence of bacteria onto fish mucus so that preventive measures can be devised to reduce infection. In vitro adhesion assay was carried out by bacterial counting. The adhesive bacteria were stained with SYBR Green Ⅰ , observed under fluorescent microscope, counted on computer display after taking pictures. The results showed that the adhesive quantity of V. alginolyticus increases with bacterial concentrations and reached equilibrium after being incubated 1 - 1.5 h;the higher adhesive quantity was achieved at 15 - 30 ℃ and acid condition; adhesion of V. alginolyticus to the mucus of foregut was not obvious variety with salinity(5 - 35) ,but adhesive quantity to the hindgut mucus enhances while salinity increases; V. alginolyticus could not adhere to intestinal mucus without salinity; adhesive quantity reduced remarkably after heat treatment of the germ(56 ℃ 5 min, 60 ℃ 1 h), which indicated that heat sensitive structure of V. alginolyticus might play an important role in adhesion. The conclusions of this paper showed V. alginolyticus could adhere to intestinal mucus of P. crocea facilely in seawater; this adhesion was remarkably affected by environmental factors, such as temperature, pH and salinity; V. alginolyticus adhered to the intestinal mucus of P. crocea by specific adhesion, some heat-sensitive structures on V. alginolyticus surface played a crucial role in adherence of the bacteria to gill mucus. The results indicated that the bacterial adhesion of V. alginolyticus to intestinal mucus of P. crocea was govemable. The results obtained are useful for the epidemic control in fish culture.
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