文章摘要
张彤晴.高效液相色谱法测定养殖水体孔雀石绿和无色孔雀石绿[J].水产学报,2007,31(5):699~703
高效液相色谱法测定养殖水体孔雀石绿和无色孔雀石绿
Determination of malachite green and leucomalachite green in aquatic water by high
投稿时间:2007-07-06  修订日期:2007-07-06
DOI:10.3724/SP.J.00001
中文关键词: 孔雀石绿  无色孔雀石绿  高效液相色谱法  养殖水体
英文关键词: malachite green  leucomalachite green  high performance liquid chromatography  aquatic water
基金项目:国家“十一五”科技支撑计划
作者单位E-mail
张彤晴 江苏省淡水水产研究所江苏南京 goldensun_001@163.com 
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       A high performance liquid chromatography method had been established for the determination of malachite green and leucomalachite green in aquatic water. Malachite green and leucomalachite green were extracted with dichloromethane, then concentrated, and dissolved with acetonitrile. Samples were chromatographed using an acetonitrile-ammonium acetate buffer mobile phase on a C18 column in line with a post column reactor filled with lead (IV) oxide and celite. The liner range was from 0.01μg•mL-1 to 1μg•mL-1 with r2 of 0.9999. The average recoveries of MG were 84.00%, 86.40%, 93.50%, and LMG were 74.00%, 89.00%, 91.80% when samples were spiked with MG and LMG of 0.1, 0.5, 1.0μg•mL-1 respectively. The detection limits of MG and LMG were 0.003μg•mL-1 and 0.004μg•mL-1 respectively. The RSDs were 4.278%-6.250% and 2.179%-6.757% respectively. The method is sensitive, accurate and suitable for the determination of trace malachite green and leucomalachite green in aquatic water. Key words: malachite green ; leucomalachite green; high performance liquid chromatography; aquatic water
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