文章摘要
马洪雨,陈松林,田永胜.我国引进条斑星鲽群体遗传多样性的AFLP分析[J].水产学报,2008,32(3):321~326
我国引进条斑星鲽群体遗传多样性的AFLP分析
Genetic diversity of introduced Barfin flounder (Verasper moseri) populations by AFLP analysis
投稿时间:2007-06-10  修订日期:2007-10-09
DOI:10.3724/SP.J.00001
中文关键词: 条斑星鲽  遗传多样性  扩增片断长度多态性  遗传分化
英文关键词: Verasper moseri  genetic diversity  AFLP  genetic differentiation
基金项目:国家高技术研究发展计划
作者单位E-mail
马洪雨 中国海洋大学 海洋生命学院山东 青岛 266003  
陈松林 中国水产科学研究院黄海水产研究所 chensl@ysfri.ac.cn 
田永胜 中国水产科学研究院黄海水产研究所  
摘要点击次数: 5731
全文下载次数: 2946
中文摘要:
       应用AFLP技术对我国条斑星鲽引进群体(烟台、大连和莱州)共63尾个体的遗传多样性及遗传变异进行分析,计算了3个群体间的遗传相似性指数和遗传距离,并构建了UPGMA系统发生树。10个引物组合在3个群体中共扩增到827个位点,大小位于50~700bp之间。每个引物组合扩增到的多态性条带在8到37条之间不等,平均为17.9个。3个群体的多态性位点比例分别为29.14%、15.60%和20.31%;Shannon’s多样性指数分别为0.1799、0.0949和0.1231;Nei’s基因多样性指数分别为0.1225、0.0658和0.0848。3个条斑星鲽群体的遗传多样性水平,烟台群体最高,莱州群体次之,大连群体最低,但总体水平均较低。烟台群体与大连群体间的遗传距离最大为0.0230,莱州群体与大连群体间的遗传距离最小为0.0129。3个群体间的遗传分化系数(Gst)为0.219,表明3个群体之间发生了一定程度的分化。
英文摘要:
      Amplified fragment length polymorphism (AFLP) technology was used to evaluate the genetic diversity of three introduced populations (Yantai, Dalian and Laizhou) of introduced barfin flounder(Verasper moseri). The genetic distances and identity index were calculated, and according to them, the UPGMA dendrogram was constructed. A total of 827 loci ranged from 50-700 bp were detected using 10 primer combinations, and 8-37 polymorphic loci were detected per primer combination. The percentage of polymorphic loci of three introduced populations of barfin flounder were 29.14%, 15.60% and 20.31%, respectively. The Shannon’s information index were 0.1799, 0.0949 and 0.1231, respectively, and the Nei’s gene diversity were 0.1225, 0.0658 and 0.0848, respectively. The total genetic diversity level of three introduced populations was low, of which YT population was the highest, LZ population was lower, and DL population was the lowest. The genetic distance between YT introduced population and DL introduced population was the highest(0.0230), whereas, the genetic distance between LZ introduced population and DL population was the lowest(0.0129). The coefficient of gene differentiation(Gst) value was 0.219, showing a certain extent of differentiation among three populations of Barfin flounder.
HTML   查看全文   下载PDF阅读器
关闭

手机扫一扫看