文章摘要
李家乐,董志国,郑汉丰.三角帆蚌三种群F1遗传差异的RAPD分析[J].水产学报,2007,31(6):848~852
三角帆蚌三种群F1遗传差异的RAPD分析
RAPD analysis on genetic variance of F1 of Hyriopsis cumingii from three populations
投稿时间:2007-11-16  修订日期:2007-11-16
DOI:10.3724/SP.J.00001
中文关键词: 三角帆蚌  地理种群  杂交F1  遗传差异  RAPD
英文关键词: Key words: Hyriopsis cumingii  geogrophical population  F1  genetic variance  RAPD
基金项目:上海市重点学科建设项目
作者单位E-mail
李家乐 上海水产大学农业部水产种质资源 jlli@shfu.edu.cn 
董志国 淮海工学院江苏省海洋生物技术重  
郑汉丰 中国水产科学研究院东海水产研究  
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中文摘要:
      
英文摘要:
      With the random amplified polymorphic DNA (RAPD) analysis method, forty ten base arbitrary primers were used to study genetic variance of 9 F1 stocks produced from hybriding three geographical populations of Hyriopsis cumingii from Dongting Lake(DD), Poyang Lake(PP) and Taihu Lake(TT). The amplified results of thirteen primers were stably and clearly endowed with abundant stock polymorphy, and each primer could gain 2-17 fragments (200-3000 bp) in the 40 primers. Proportion of polymorphy was from 55% to 100%. AMOVA of 9 stocks indicated that 7.15% genetic variance was from intra stock and 92.85% from inter stock. Fixation index (Fst), the three lower were DP and PD(0.013),TP and TT(0.020) and TP and TD(0.026),the 4 higher were as follows: PT and DT(0.165)>TT and PT(0.153) >〖JP〗TD and PT (0.150)> DD and PT(0.137). Inter stock gene diversity (π) was as follows:TP(0.335)>TD(0.317)>TT(0.308)>PD(0.300)>DP(0.298)>PP(0.297)>DT (0.291)>PT(0.291)>DD(0.269).
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